However, consistent with previous studies (Fisher et al., 2012), there was no obvious change in the serum level of FGF21 throughout the period of cold exposure (Figure 1J). Although there was a mild increase in serum levels of FGF21 after 6 hr and 1 day of cold exposure, the change...
Visual signs were on the walls surrounding the pool to assist the animals. The platform was placed in the southwest quadrant, and was immobile throughout the trial until the probe was administered. The interval between the trials was at least 10 min. On Day 1–3, each mouse was placed ...
serum FGF21 level was the highest before beta-conglycinin intake and gradually and significantly decreased throughout the 2-h experimental period (P This study reveals that although serum FGF21 levels are not increased by a single or short-term intake of beta-conglycinin, the 螖 basal FGF21 lev...
the FGF21 response whereas inulin did not (Fig.2a, b). Conversely, when protein was provided in sufficient quantity, fiber supplementation had no impact on FGF21 levels (Fig.2a, b). We tested whether the cellulose and inulin response on the PR diet was dose-dependent by supplementing both...
Both plasma and hepatic Fgf21 levels are increased in Crtc2 liver-specific knockout mice, as a result of the reduced miR-34a expression regulated by Creb/Crtc2 and the induction of Sirt1 and Pparα. Ectopic expression of miR-34a reverses the metabolic changes in knockout liver. We suggest...
More particularly, FGF21 effects kick in at higher plasma levels as compared to GLP-1 effects. In addition, at higher levels, GLP-1 is known to have adverse effects, e.g., nausea and vomiting. Taken together, this implies a potential risk of GLP-1-mediated adverse effects when ...
As a non-canonical fibroblast growth factor, fibroblast growth factor 21 (FGF21) functions as an endocrine hormone that signals to distinct targets throughout the body. Interest in therapeutic applications for FGF21 was initially sparked by its ability to correct metabolic dysfunction and decrease bod...
Both genes demonstrated increased expression levels when WT MEFs were treated with erastin. However, the upregulation of FGF21 expression was modest in Bach1−/− MEFs, indicating that FGF21 expression was dependent on BACH1 during ferroptosis (Figure 3B). In contrast, the degree of up...
2.5. Measurement of mRNA steady-state levels Total RNA was isolated using RNeasy spin columns from GE Healthcare (Madrid, Spain). cDNA synthesis was performed with SuperScript™VILO™ cDNA Synthesis Kit (Thermo Fisher Scientific). Detection of amplification products was carried out using SYBR Gr...