Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms fibroblast growth factor 21, Fgf21, FGF-21Publications SpeciesSample TypeTitle mouse serum Sci Adv Mitochondrial proteostasis ...
FGF21 ELISA Plasma was isolated from whole blood centrifuged at 3000 × gfor 15 min at 4 °C. FGF21 concentration was measured using the Mouse and Rat FGF-21 ELISA Kit (Biovendor, Brno, Czech Republic) following the manufacturer’s protocol. ...
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The animal study protocol was approved by the Ethics Committee of Xi’an Medical University (2015KTCQ03-03). 2.9 ALT and AST assays ALT and AST activities in mouse serum were measured using the colorimetric assay kits according to the instruction. Briefly, the serum samples were mixed with ...
kit, according to the manufacturer’s protocol (R&D Systems). Animal experiments using these mice were approved by and performed according to the guidelines of the Committee of Experimental Animal Research of Gunma University. Preparation of neonatal rat cardiac myocytes ...
AnimalUseandCareCommittee(ProtocolNo.12225). PLOSONE | 1 November2012 | Volume 7 | Issue 11 | e49977 FGF21RequiresbklothotoActInVivo PLOSONE | 2 November2012 | Volume 7 | Issue 11 | e49977 FGF21RequiresbklothotoActInVivo Figure1.ExpressionofFGFR/Klothoreceptorcomponents(A–F),KLBprotein...
The staining protocol was following: 1) the tissue sections were hydrated: the paraffin of the slides was melted and replaced by xylene and alcohol, and the slides were immersed for 30 s with agitation by hand in H2O; 2) the slides were dipped into a Coplin jar containing hematoxylin and ...
Enzyme Linked Immunosorbent Assay(ELISA). The serum insulin and norepinephrine concentrations of rats were measured by using ELISA kits (E-EL-R2466c and E-EL-0047c, respectively; Elabscience, China). Immunohistochemistry and Whole Section Visualization. The immunohistochemistry protocol for NG ...
RNAs collected from FACS-sorted EGFP-negative and positive cells were reversed transcribed using High-Capacity Reverse Transcription (Life Technologies) and the linear preamplification stage was carried out with the TaqMan PreAmp Master Mix Kit Protocol (4366128, Applied Biosystems). Real-time PCR was...
Where indicated, plasma FGF21 concentration was also determined in duplicate by a mouse-specific ELISA kit (EMD Millipore, Billerica, MA) according to the manufacturer’s protocol in an effective range of 50–12000 pg/ml. The coefficient of variation was less than 10% within a same analysis a...