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It should be taken into account that the use of viral vectors presents several limitations such as rapid clearance and triggering the immune response that diminish the effectiveness of viral therapy (for example, in the review by ref. 52). Despite being aware of these limitations, we want to ...
Where indicated, plasma FGF21 concentration was also determined in duplicate by a mouse-specific ELISA kit (EMD Millipore, Billerica, MA) according to the manufacturer’s protocol in an effective range of 50–12000 pg/ml. The coefficient of variation was less than 10% within a same analysis a...
FGF21 ELISA Plasma was isolated from whole blood centrifuged at 3000 × gfor 15 min at 4 °C. FGF21 concentration was measured using the Mouse and Rat FGF-21 ELISA Kit (Biovendor, Brno, Czech Republic) following the manufacturer’s protocol. ...
we also observed a 10-fold peak increase in total FGF21 with acute alcohol when using the same ELISA kit. Thus, alcohol bingeing (e.g. 0.9 g/kg) may elicit exaggerated FGF21 secretion relative to moderate drinking, which could be relevant to the pathogenesis of alcohol abuse if FGF21 ...
Blots were developed using an ECL kit (Amersham Biosciences, Piscataway, NJ). The chemiDoc™XRS + with Image Lab™Software (BIO-RAD, USA) was used for development. 2.10. Enzyme-linked immunosorbent assays (ELISA) The protein levels of tau and β-amyloid1−42 in the supernatants of ...
Breast cancer and lifestyle factors: umbrella review. Hematol Oncol Clin North AM. 2024;38(1):137–70. Article PubMed Google Scholar Brown KA. Metabolic pathways in obesity-related breast cancer. Nat Rev Endocrinol. 2021;17(6):350–63. Article PubMed PubMed Central Google Scholar Crudele...
C-terminal 12 aa of the standard form (5). Mature human FGF-21 shows 81% aa identity to mouse FGF-21, and is known to be active on mouse cells (4, 6). The FGF-19 subfamily is considered endocrine in nature. All three subfamily members impact some aspect of metabolism, all three ...
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During analysis of the ELISA data the blank values were subtracted from the experimental values to account for non-specific absorbance detected in the plate wells. Plasma ethanol (EtOH) concentrations were determined using a commercially available kit (EnzyChrom). In both cases the manufacturer’s ...