Fatty acid oxidation (FAO)-driven H2O2 has been shown to be a major source of oxidative stress in several tissues and disease states. Here, we established that the mitochondrial flavoprotein long-chain acyl-CoA dehydrogenase (LCAD), which catalyzes a key step in mitochondrial FAO, directly produ...
A low CACT:CPT1A ratio in immune cells from T2D subjects indicates altered mitochondrial function and coincides with the preference of these cells to generate ATP through glycolysis rather than fatty acid oxidation. However, glycolysis was not critical for Th17 cytokines. Instead, β oxidation ...
The importance of fatty acid (FA) metabolism in cancer is well-established, yet the mechanisms underlying metabolic reprogramming remain elusive. Here, we identify tetraspanin CD37, a prognostic marker for aggressive B-cell lymphoma, as essential membrane-localized inhibitor of FA metabolism. Deletion ...
We used the Seahorse XF Mito Fuel Flex Test to monitor the dependency on fatty acids as a source of energy in LSK cells from LPS (16 hours) treated animals. Results confirm an increased dependency on fatty-acid oxidation (FAO) compared to control LSK cells (Fig.2d). To determine the ...
Didymin switches M1-like toward M2-like macrophage to ameliorate ulcerative colitis via fatty acid oxidation. Pharmacol Res. 2021;169: 105613. Article CAS PubMed Google Scholar Dai H, et al. Crystallographic structure of a small molecule SIRT1 activator-enzyme complex. Nat Commun. 2015;6:7645...
13C isotopomer tracing experiment and Seahorse XF analysis were undertaken to determine the activity of fatty acid oxidation (FAO). qRT-PCR was performed to evaluate the expression of essential FAO genes including CPT1A. NPC tumor tissue microarray was used to investigate the prognostic role of ...
Type 2 alveolar epithelial (AT2) cells of the lung are fundamental in regulating alveolar inflammation in response to injury. Impaired mitochondrial long-chain fatty acid β-oxidation (mtLCFAO) in AT2 cells is assumed to aggravate alveolar inflammation i
Surplus EPA resulted from an imbalance between DHA and EPA in the enriched nauplii probably caused an adverse effect on the seahorse juveniles. This study suggests that DHA and EPA requirement of the lined seahorse juveniles is roughly 32% of total fatty acid, and the optimal DHA/EPA ratio ...
presence of etomoxir, further demonstrating that avocatin B accumulates in mitochondria.Since avocatin B is a lipid that targets mitochondria and that mitochondria can oxidize fatty acids for energy, we next assessed the impact of avocatin B on fatty acid oxidation, using the Seahorse Bioanalyzer....
fatty acid oxidation (FAO)51, a process shown to be essential in certain malignancies such as MYC-overexpressing breast cancer52; (4) the preferred source of palmitate for cancer cells could be the endogenously synthesized fatty acid by FASN. However, to our surprise, none of these four ...