Solution:The Seahorse Bioscience XFp Extracellular Flux Analyzer simultaneously measures mitochondrial respiration and glycolysis, the two major energy-producing pathways in a cell, in a cell culture microplate, in real time. XF technology works with a variety of cells offering physiologically relevant cel...
IL-2-treated cells secrete more lactate and protons, as measured by extracellular acidification rates in a Seahorse Assay. IL-2-treated cells also express more of the phosphorylated, inactive form of pyruvate dehydrogenase (PDH). PDH controls the metabolic fate of pyruvate, driving it towards ...
Interestingly, extensive investigation of the candidate genes of the oxidative or glycolytic pathways showed a differential expression between the 4-1BB and CD28 CAR T cells that was consistent with the Seahorse assay results. Similarly, the ratio of mitochondrial mass to cell mass and the ...