美国海马细胞能量代谢实时测定仪/生物能量代谢测定仪XFe24/96(Seahorse XFe Extracellular Flux Analyzer)——2009年全球创新技术产品Top10!美国海马生物科学利用细胞外流量(Extracellular Flux,XF)检测专利技术,发明了业界第一款海马细胞能量代谢实时测定仪/生物能量代谢测定仪XFe24/96,是进行细胞代谢分析、氧呼吸测定、...
安捷伦 Seahorse XFe24 分析仪以 24 孔板形式测量活细胞的耗氧率 (OCR) 和细胞外酸化率 (ECAR)。 OCR 和 ECAR 是线粒体呼吸和糖酵解以及 ATP 生成速率的关键指标,综合这些检测结果可在系统水平表征培养细胞和体外样品的细胞代谢功能。 XF 分析仪
美国海马生物科学利用细胞外流量(Extracellular Flux,XF)检测ZL技术,发明了业界**款海马细胞能量代谢实时测定仪/生物能量代谢测定仪XFe24/96,是进行细胞代谢分析、氧呼吸测定、药物代谢分析、线粒体有氧代谢和糖酵解等功能的**分析工具。 详细介绍 Seahorse XFe细胞能量代谢分析仪 ...
This chapter summarizes a relatively new and exciting approach based on the Seahorse Extracellular Flux Analyzer, which takes real-time measurements of oxidative phosphorylation and glycolysis in living cells. These bioenergetic profiles are then compared with steady-state levels of cellular adenosine ...
Meanwhile, we employed the exaggerated extracellular matrix deposition assay to investigate the effect of MFSD7C depletion on fibrosis. The HuLEC-5A cells were cultured in the presence and absence of Fer-1, then conditioned mediums were collected and applied in normal human lung fibroblasts (nHLF...
GlycolysisStressTestKitUserGuide3ProductDescriptionTheSeahorseXFGlycolysisStressTestisthestandardassayformeasuringglycolyticfunctionincells.Bydirectlymeasuringtheextracellularacidificationrate(ECAR;Figure1),theSeahorseXFGlycolysisStressTestprovidesastandardandcomprehensivemethodtoassessthekeyparametersofglycolyticflux:Glycolysis,...
Seahorse Xfe 24 Extracellular Flux Analyzer-Based Analysis of Cellular Respiration in Caenorhabditis elegans. Curr Protoc Toxicol. 2015; 66:25 7 1-15. Epub 2015/ 11/03. doi: 10.1002/0471140856.tx2507s66 PMID: 26523474; PubMed Central PMCID: PMC4632645.Luz, A. L., Smith, L. L.,Rooney...
cruzi. Here, we evaluate the Seahorse XFe24 extracellular flux platform as an alternative method to assess mitochondrial bioenergetics in isolatedT. cruziparasites. We report optimized assay conditions used to perform mitochondrial stress tests with replicative life cycle stages ofT. cruziusing the XFe...
using the Agilent Seahorse XFe24 or XF24 Extracellular Flux Analyzer, it is essential to first characterize a specific cell type with respect to its metabolic activity under basal and maximal respiration (OCR) and extracellular acidification (ECAR). The Seahorse XF Cell Energy Phenotype Kit can ...
This command is required for the XFe24 Analyzers in every assay, but may be added to Instrument Protocol for any analyzer. • Measure: The amount of time to record the flux of analytes in the transient microchamber once the sensor cartridge probes are lowered following a Mix (or Wait) ...