Fatty acid oxidation (FAO)-driven H2O2 has been shown to be a major source of oxidative stress in several tissues and disease states. Here, we established that the mitochondrial flavoprotein long-chain acyl-CoA dehydrogenase (LCAD), which catalyzes a key step in mitochondrial FAO, directly produ...
A low CACT:CPT1A ratio in immune cells from T2D subjects indicates altered mitochondrial function and coincides with the preference of these cells to generate ATP through glycolysis rather than fatty acid oxidation. However, glycolysis was not critical for Th17 cytokines. Instead, β oxidation ...
Here, through large-area hyperspectral SRS imaging and subsequent single-cell analysis, we identified a stable metabolic switch from glucose and glycolysis dependent to FA uptake and fatty acid beta-oxidation (FAO) dependent anabolic and energy metabolism in cisplatin-resistant OC cells. By coupling ...
Herein we demonstrate that resistance to ven/aza occurs via upregulation of fatty acid oxidation (FAO), which occurs either due to RAS pathway mutations or as a compensatory adaptation in relapsed disease. Utilization of FAO obviates the need for amino acid metabolism, thereby rendering ven/aza ...
miR-30 enhances exogenous fatty acid β-oxidation and promotes oxidative stress and EC dysfunction. A Representative Seahorse tracing of oxygen consumption rate (OCR) in control and miR-30e over-expressing ECs. Basal and maximal respiration of exogenous, but not endogenous FA, was enhanced in miR...
fattyetomoxirnadphoxygenoxidationatp InhibitionoffattyacidoxidationbyetomoxirimpairsNADPHproductionandincreasesreactiveoxygenspeciesresultinginATPdepletionandcelldeathinhumanglioblastomacells☆LisaS.Pikea,AmyL.Smifta,NicoleJ.Croteaub,DavidA.Ferricka,MinWua,⁎aSeahorseBioscience,NorthBillerica,MA01862,USAbDepartmentof...
Didymin switches M1-like toward M2-like macrophage to ameliorate ulcerative colitis via fatty acid oxidation. Pharmacol Res. 2021;169: 105613. Article CAS PubMed Google Scholar Dai H, et al. Crystallographic structure of a small molecule SIRT1 activator-enzyme complex. Nat Commun. 2015;6:7645...
13C isotopomer tracing experiment and Seahorse XF analysis were undertaken to determine the activity of fatty acid oxidation (FAO). qRT-PCR was performed to evaluate the expression of essential FAO genes including CPT1A. NPC tumor tissue microarray was used to investigate the prognostic role of ...
presence of etomoxir, further demonstrating that avocatin B accumulates in mitochondria.Since avocatin B is a lipid that targets mitochondria and that mitochondria can oxidize fatty acids for energy, we next assessed the impact of avocatin B on fatty acid oxidation, using the Seahorse Bioanalyzer....
fatty acid oxidation (FAO)51, a process shown to be essential in certain malignancies such as MYC-overexpressing breast cancer52; (4) the preferred source of palmitate for cancer cells could be the endogenously synthesized fatty acid by FASN. However, to our surprise, none of these four ...