AssayResultReference interval PTT-LA 67 s 34–50 s Staclot-LA; hex-phase phospholipid APTT reagent 54 s Difference 13 s 8 s difference DRVVT 47.5 s 30.9–41.5 s DRVVT with high phospholipid 33 s DRVVT ratio 1.4 < 1.2 What is the correct interpretation of these patient’s results? A...
The factor X processing cleavage site lies between residues Arg194-Ile195 [70, 71], releasing Xa, which in turn activates the precursor coagulation factor V, another plasma glycoprotein with three distinct cleavages, and forms the protease complex Xa:Va. Factor V can also be activated by ...
Factor X activity (FXa) was determined using the ACTICHROME Factor X ®chromogenic activity assay (American Diagnostica Inc., #880) as per manufacturer's instructions. Histology. SRA segments were collected at the end of experiments into optimal cutting compound (ProSciTech), frozen in cold...
Factor VIII is a cofactor in the coagulation cascade but has no intrinsic enzymatic activity. Therefore, the concentration of Factor VIII is made the rate limiting step in a reaction that generates Factor Xa - the First Stage of the Two Stage Assay. The Second Stage determines the amount of ...
A 1-stage APTT-based Factor assay is used for the measurement of Factors VIII, IX, XI and XII. It can also be used for assaying Factors X, V and II [Prothrombin] although a PT-based assay is more commonly used. Factor XII can also be assayed using this technique as can Pre-Kallikre...
(Fig.6d). Electrophoretic mobility shift assay (EMSA) experiments further verified that TcBR-C-z2 directly binds to theTcAK1andTcAK2promoter regions to regulate their expression (Fig.6e). Our interpretation is that the opposite hormonal regulation ofTcAK1andTcAK2was primarily mediated by TcBR-C-...
White lupin seed is a unique legume rich in protein and fiber contents, as well as phytochemicals with health potential that contributes to a reduced risk of dyslipidemia, obesity and intestinal dysfunction. This study was focused on the effect of the ye
To determine if PLAGL1 could play a role in the ability of HTR-8/SVneo cells to mimic endothelial cell tube-like structure formation, we performed a tube formation assay. Ten hours after plating the cells transfected with the PLAGL1 siRNA, we see a significant decrease in cord formation,...
The invention relates to factor X analogues which have a modification in the area of the naturally occurring factor Xa activating cleavage site, said modification representing a processing site of a protease which does not naturally cleave in this area of the factor X sequence. The invention also...
Furthermore, although the assay is suitable to quantify the coagulation property of cell therapeutic products, it might fail to reflect their behaviors in vivo, as many environmental milieus, such as inflammation, hypoxia, or Ca2+ in serum, might immensely change their TF-mediated coagulant ...