Linkage analysis combined with whole-exome sequencing identifies a novel prothrombin (F2) gene mutation in a Dutch Caucasian family with unexplained thrombosisdoi:10.3324/HAEMATOL.2019.232504René MulderTon LismanJoost C.M. MeijersJames A. Huntington...
急性髓系白血病SRSF2基因突变患者临床病理特征及预后生存分析
Mutation analysis of TINF2 in patients with genetically uncharacterized DC As previously described mutations in TINF2 affected amino acids (aa) 280 and aa 282 in exon 6, we initially screened this exon in all uncharacterized index patients present in the DCR. Of the 292 families available in th...
Survival analysis revealed that the S2 PDAC had a median progress-free survival (PFS) time and overall survival (OS) time that were significantly shorter than the S1 PDAC (6.6 versus 11.2 months, log-rankP < 0.0001 and 11.4 versus 13.3 months, log-rankP = 0.041, respectively; Fig...
The analysis was performed using the 2−△△CT method. 2.5 Western blot analysis RIPA buffer (Solarbio, Beijing, China) with proteinase inhibitors was used to isolate proteins from PC cells. The BCA kit (Beyotime) was used to determine the concentration of proteins. 20 μg protein were ...
Interestingly, a set of 67 genes whose expression increased during EMT20 tended to have greater expression in MEF2B-V5 cells than in untransfected cells (gene set enrichment analysis21,22 false discovery rate 0.105; Supplementary Fig. 6). Among these genes were the well-known EMT inducers TGF...
mutation was not frequently occurred in CMML patients and might associated with poor prognosis.It might be a pratically diagnostic maker and therapeu— tic target in CMML. 【Key words】Gene,SRSF2;DNA mutational analysis;Leukemia,myelomonocytic,chronic ...
PB1-F2 is a small, 87- to 90-amino-acid-long protein encoded by the +1 alternate open reading frame of the PB1 gene of most influenza A virus strains. It h... ZT Varga,A Grant,B Manicassamy,... - 《Journal of Virology》 被引量: 385发表: 2012年 A single N66S mutation in ...
Atm mutation leads to the disorder known as ataxia-telangiectasia. The blood disorders Sickle cell anemia and thalassemia are caused by HBB gene mutations, while Wilms' tumors, WAGR syndrome and Denys-Drash syndrome are associated with mutations of the WT1 gene. Jervell and Lange-Nielsen syndrome,...
In this assay, Cas9 stimulates homologous recombination with a homology arm cassette (HAC) that contains sequences homologous to the specific genomic location targeted by the gRNA flanking a blasticidin-resistance gene. Targeted insertions of the HAC can be identified through PCR analysis of DNA from...