Normal distribution was determined by a quantile-quantile plot and data were analysed by unpaired 2-tailed Student’s t test or, in the case of multiple comparisons, one-way analysis of variance (ANOVA) followed by post hoc analysis using Tukey’s multiple comparisons test. R2-values were calc...
To generate a pathogenic mouse-adapted strain of SARS-CoV-2 we used a clinical isolate of the SARS-CoV-2 Alpha variant (Pango Nomenclature B.1.1.7) [29] as a parental strain for mouse adaptation. This strain contained the N501Y mutation within the Spike protein which had, at the time, ...
In a test of nonviral gene therapy, Yamamoto et al. electroporated a GDNF plasmid into tibialis anterior and gastrocnemius every 2 weeks beginning at 9 weeks of age. GDNF was present in both the muscle and MNs. Survival was not improved, but motor function did respond to therapy.155 Moren...
modified PT andAPTTassays.348,350The degree of correction of the long clotting times of factor-deficient plasma by the plasma being tested is proportional to the activity of the specific factor being analyzed for in the test sample. Chromogenic assays are also available for some coagulation ...
(2) For each pair of a TF and a phenotype, we calculated the odds ratio and the P value of the one-tailed Fisher’s exact test on SNPs with candidate ASBs considering two binary features: whether the SNP is associated with the phenotype, and whether the SNP is included in ASB candida...
mutation at a random position within the selected binding site for each motif. PR curves and AUC values were computed to compare the performance of the different methods (Figure4and Table3). ROC curves for this experiment can be obtained from the additional file2: "ROC curves". The ROC and...
NR2F2 regulation of interstitial cell fate in the embryonic mouse testis and its impact on differences of sex development Article Open access 29 April 2025 The construction of a testis transcriptional cell atlas from embryo to adult reveals various somatic cells and their molecular roles Article...
Deregulation of miRNAs has been linked to tumor development and progression, and a growing number of miRNAs have been described as candidate oncogenes or tumor suppressors [2]. In breast cancer, distinct expression profiles of miRNAs have been associated with specific molecular subtypes and ...
V. cholerae C6706 with pPDE showed significantly increased motility compared to the vector control (Additional file 2: Figure S1A). Importantly, V. cholerae C6706 with pPDEmut, which contains an E170A mutation that renders VieA enzymatically inactive [10], showed motility equal to the vector ...
0.05, **P < 0.01, ***P < 0.001, Student’st-test.cTM promoter luciferase reporter assay. ERG cDNA expression plasmid (ERG) or empty expression plasmid (pcDNA) were co-transfected with TM promoter-luciferase constructs (TM wild type (WT), TM mutant 1 or TM mutant 2) or a pGL...