F-type ATPases are highly conserved enzymes used primarily for the synthesis of ATP. Here we apply mass spectrometry to the F1FO-ATPase, isolated from spinach chloroplasts, and uncover multiple modifications in soluble and membrane subunits. Mass spectra of the intact ATPase define a stable lipid...
Furthermore, several studies focused on the assembly process of bacterial F-type ATPases. For instance, the sodium-driven Na+-F1FO-ATPase of Acetobacterium woodii has an unusual c-ring, the assembly of which has been shown to require the assembly factor AtpI, which could not be replaced ...
The rotary proton- and sodium-translocating ATPases are reversible molecular machines present in all cellular life forms that couple ion movement across membranes with ATP hydrolysis or synthesis. Sequence and structural comparisons of F- and V-type ATPases have revealed homology between their catalyt...
Common patterns and unique features of P-type ATPases: a comparative view on the KdpFABC complex from Escherichia coli. Mol. Membr. Biol. 24:375-386.Bramkamp M, Altendorf K, Greie J-C. 2007. Common patterns and unique features of P-type ATPases: a comparative view on the KdpFABC ...
V. (2007). Inventing the dynamo machine: the evolution of the F-type and V-type ATPases. ...
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Changes in the primary and quarternary structure of vacuolar and archaeal type ATPases that accompany the prokaryote-to-eukaryote transition are analyzed. The gene encoding the vacuolar-type proteolipid of the V-ATPase fromGiardia lambliais reported.Giardiahas a typical vacuolar ATPase as observed from...
While TccC3 HVR ADP-ribosylates actin at T148, TccC5 HVR ADP-ribosylates Rho GTPases at E61 and E63. Together, their activities promote aberrant actin polymerization, cause actin clustering and aggregation, and ultimately cell death18,21. However, the molecular mechanism of action of these ...
On the basis of our results, various mechanistic pump cycle models were derived from the general Post-Albers scheme of P-type ATPases and discussed in the framework of the experimental evidence to propose a possible molecular pump cycle for KdpFABC. 展开 ...