Exosome treatment of cell lines and viability assays Cell lines were cultured in exosome depleted media in 24-well culture plates. Cells were treated with control exosomes/test exosomes (108 particles/ml media) for 4 days. Following this treatment, cells were plated in 96-well plates and assayed...
Patients who received TIL treatment had a considerably longer median PFS (7.2 months) than those who received anti-PD-1 treatment (3.1 months). Nevertheless, the patients who had TIL therapy experienced a significantly greater incidence
Moreover, exosome treatment alleviated SCI-induced pain behaviors and reduced pain-associated proteins (BDNF, TRPV1, and Cav3.2). Exosomal miRNA analysis revealed several promising therapeutic miRNAs. The cell culture study also confirmed the neurotrophic effect of HucMSCs-EX.#Implantation of HucMS...
代理Sino Biological抗体/蛋白质/抗原/多肽已认证 品牌介绍 北京义翘神州科技股份有限公司(Sino Biological Inc.)是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司,主要业务包括重组蛋白、抗体、基因和培养基等产品,以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研...
[54]. The 3D culture system improved the functionality of MSC-Exo for the treatment of periodontitis, and 3D-Exo exhibited greater enrichment of miR-1246, which inhibits the expression of Nfat5 of Th17, and in turn treats periodontitis more effectively [55]. Shen et al. loaded the exosomes...
For example, experiments examining the secretion rate and/or concentration of exosomes into cell culture media following treatment of cells with broad spectrum deubiquitylase or E3 ligase inhibitors may provide novel information on this aspect. Defining a valid ubiquitylation motif has been the subject...
and incubated at 37 °C for 1 h. Cells were then treated by trypsin to remove antibodies still present at the cell surface (= stripping, Fig.4b) before quantitative analysis by flow cytometry. This treatment efficiently removed antibodies remaining at the cell surface, since the signal was...
Non-small cell lung cancer (NSCLC) remains an ongoing health concern, with poor treatment options and prognosis for many patients. Typically, individuals with lung cancer are detected at the middle and terminal stages, resulting in poor medical results d
When it comes to clinical analysis, ExoSCOPE realized efficient distinction of responders and non-responders within 24 h after treatment [126]. Different from SPR, using metal film as the substrate, LSPR generally uses nanoparticles or discs located on a glass substrate. Liu et al. decorated ...
On the contrary, a reduction in viability of almost 50% was observed for irradiated MSCs after treatment with HGN-loaded exosomes derived from MSCs. Very similar results were obtained using a fluorescence inverted microscope to quantify live/dead cells after exposure to MSC-derived exosomes followed...