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Size exclusion chromatography (SEC) has become the method of choice for rapid isolation of relatively pure EVs from plasma, yet it has technical limitations for certain downstream applications. The recently released exoEasy kit (Qiagen) is a new membrane affinity spin column method for the isolation...
(11–13 min), which has been reported to be around 5 nm in size43. Only pRNA-3WJ-cholesterol/GDENs group showed a peak in 5 min fraction, indicating the RNA colocalized with GDENs as it passed through the column. A significant FRET peak compared to control groups confirms the ...
The concentrate (500 μL) was applied to the SEC column (qEV original/70 nm, Izon, Christchurch, New Zealand) following the column equilibration procedure according to the manufacturer’s protocol [22]. The sample was eluted with PBS. Twenty fractions of the 500 μL solution pass-through ...
In this method, vesicles are fractionated as they pass through a column filled with porous beads based on size. Thus, smaller MSC-EVs will be eluted first, whereas the bigger vesicles will be retained longer [42]. One of the advantages of SEC is that it uses mild conditions, allowing the...
The use of size exclusion chromatography (SEC) to isolate exosomes from other EVs based on size is performed the same way as if one wanted to separate proteins of different sizes. That is, a column is packed with a porous stationary phase in which small particles can penetrate. This penetrat...
Human tRNASec associates with Hela membranes, cell lipid liposomes and synthetic lipid bilayers. RNA 2012, 18, 2260–2268. [CrossRef] [PubMed] 13. Araki, W.; Tamaoka, A. Amyloid beta-protein and lipid rafts: Focused on biogenesis and catabolism. Front. Biosci. 2015, 20, 314–324. [...
The column was washed sequentially with 70% acetonitrile, 100% acetonitrile, and 50 mM ammonium bicarbonate. The samples were applied and eluted with cyano-4-hydroxycinnamic acid (CHCA) (Sigma, St. Louis, MO, USA) dissolved in 70% acetonitrile and 2% formic acid onto the MALDI plate (Opti-...