Article PubMed PubMed Central Google Scholar Mathieu, M., Martin-Jaular, L., Lavieu, G. & Théry, C. Specificities of secretion and uptake of exosomes and other extracellular vesicles for cell-to-cell communication. Nat. Cell Biol. 21, 9–17 (2019). Article CAS PubMed Google Scholar...
doi:10.1007/s00018-018-2773-4 (Open in a new window)PubMed (Open in a new window)Web of Science ®(Open in a new window)Google Scholar Martins TS, Catita J, Rosa IM, Silva O, Henriques AG. Exosome isolation from distinct biofluids using precipitation and column-based approaches. ...
(1 µg per 2.5 × 105cells) provided in the kit. DNA was released from the antibody-DNA complex by proteinase K and purified through the specifically designed Fast-Spin Column provided in the kit. Eluted DNA was amplified with primers specific for HIV-1 LTR encompassing ZFP binding...
Size exclusion chromatography (SEC) has become the method of choice for rapid isolation of relatively pure EVs from plasma, yet it has technical limitations for certain downstream applications. The recently released exoEasy kit (Qiagen) is a new membrane affinity spin column method for the isolation...
PubMed PubMed Central Google Scholar Wang, Q. et al. Grapefruit-Derived Nanovectors Use an Activated Leukocyte Trafficking Pathway to Deliver Therapeutic Agents to Inflammatory Tumor Sites. Cancer Res 75, 2520–2529 (2015). Article CAS Google Scholar Guo, P. The emerging field of RNA nanote...
The concentrate (500 μL) was applied to the SEC column (qEV original/70 nm, Izon, Christchurch, New Zealand) following the column equilibration procedure according to the manufacturer’s protocol [22]. The sample was eluted with PBS. Twenty fractions of the 500 μL solution pass-through ...
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The column was washed sequentially with 70% acetonitrile, 100% acetonitrile, and 50 mM ammonium bicarbonate. The samples were applied and eluted with cyano-4-hydroxycinnamic acid (CHCA) (Sigma, St. Louis, MO, USA) dissolved in 70% acetonitrile and 2% formic acid onto the MALDI plate (Opti-...