Exhaustion of CD4 + T cells coincides with expression of interferon (IFN) response genes [3], referred to as the IFN signature. The development of the IFN signature has been suggested to predate RA [4]. Objectives We investigated how smoking affect the CD4 + T cell population in peripheral...
T-cell exhaustion (CD8PD-1/CTLA-4) and treatment-induced depletion of regulatory T-cells (CD4Foxp3/CTLA-4) was seen in tumor or blood in 5/5 patients with clinical benefit, but only in one non-responder. Tumor lymphocyte infiltration was 0.17%. Only two non-responders had PD-L1 ...
TIM-3 is expressed by exhausted T cells in the settings of chronic infection and cancer. Tumor-infiltrating macrophages and dendritic cells also express TIM-3. LAG3 is primarily expressed by activated CD4+ T cells, CD8+ T cells, FoxP3+ T regulatory cells (Tregs), and NK cells. 2B4 i...
在一些ATACseq实验(图S6,B和C)和所有ChIP-seq实验中,CD8 + T细胞 通过使用抗CD4磁性选择珠的Miltenyi阴性选择分离,并遵循制造商的方案。 13、胞内细胞因子染色 将CAR-T细胞和143B细胞按1:1的E:T (Effector:Target)比例接种于含有1x莫能菌素(EBioscience)和1ml CD107a抗体(Clone H4A3,BioLegend)的T细胞培养...
The percentages of OX-40+, HLA-DR+, and CD25+ cells in CD4+CXCR5+ T cells after different stimuli are shown. Each symbol represents an individual donor, and horizontal bars indicate the median. *p < 0.05, **p < 0.01, ***p < 0.001. Tim-3 and PD-1 blockade restores the function of...
T cells can acquire a broad spectrum of differentiation states following activation. At the extreme ends of this continuum are short-lived cells equipped with effector machinery and more quiescent, long-lived cells with heightened proliferative potential
cells in the tumor microenvironment and during human immunodeficiency virus (HIV) infection. TIM-3 is expressed by exhausted T cells in the settings of chronic infection and cancer. Tumor-infiltrating macrophages and dendritic cells also express TIM-3. LAG3 is primarily expressed by activated CD4+...
Fc–IL-4 had a negligible effect on induction of type 2 polarization in CD4+ or CD8+ T cells (Extended Data Fig. 2h–j). To explore whether Fc–IL-4 alters the transcriptomic profiles of TILs, single-cell RNA sequencing (scRNA-seq) was performed on sorted tumour antigen-specific Thy1.1...
cells from all 47 tumor samples, showing the formation of nine main clusters. Each dot corresponds to a single cell, color coded by cell cluster. d, Proportion of each CD4+ subtype in all CD4 T cells from pretreatment (pre-rx) and post-treatment (post-rx) tumors. Data presented as...
Despite the critical roles of Th1-polarised CD4+ T cells in cancer immunosurveillance, the translation of their potential to clinical use remains challenging. Here, we investigate the clinical relevance of circulating antitumor Th1 immunity in non-small