Circulating tumor cells provide a source of noninva-sively and sequentially sampled invasive cancer cells suitable for propagation in vitro. We review the advantages and challenges associated with ex vivo culture of tumor cells circulating in the blood of patients with cancer. The blood-borne spread...
前软骨模型允许1)分析软骨细胞介导的软骨组织周转改变,2)可视化软骨ECM组合,和3)软骨细胞直接在组织中重新排列。分析这些改变对刺激或治疗的反应在软骨生物学的各个方面非常重要,并补充在分离的软骨细胞体外实验,或在实验条件的活体动物中更复杂的模型更难控制。 软骨外植植物提供了一种翻译和易于访问的方法,用...
Mit Fluoreszenzmarkierung, können wir diese ex vivo Kultur zu entwickeln Vorläuferzellen und deren Differenzierung in Herzmuskelzellen zu schlagen oder Myotuben i überwachenn Echtzeit. Darüber hinaus kann dieser ex vivo-Kultursystem verwendet werden, um Rollen von zell autonomer Mikroumgebun...
398 Purpose: Cell cultures are widely used in cancer research though correlation with in vivo research is often poor. We propose an ex-vivo model of human tissue cultures that more closely resemble the in vivo three-dimensional environment to evaluate biological mechanisms present in pathological con...
We then compared the therapeutic potential of early versus late harvested CART19 in a murine xenograft model of ALL and showed that the anti-leukemic activity inversely correlated with ex vivo culture time: day 3 harvested cells showed robust tumor control despite using a 6-fold lower dose of ...
[Methods in Molecular Biology] Malaria Volume 923 || Ex Vivo Culture of Plasmodium Vivax and Plasmodium Cynomolgi and In Vitro Culture of Plasmodium Knowle... Background Plasmodium knowlesi, a simian malaria parasite, has become the main cause of malaria in Sarawak, Malaysian Borneo. Epidemiologica...
(PMVEC) or cytokines alone could rescue hematopoietic cells with in vitro colony-forming activity, in vivo radioprotective capacity, and long-term repopulating potential.ResultsPMVEC coculture supported the recovery of fourfold and 80-fold greater numbers of total cells and colony-forming cells (CFC)...
Ex-vivo slice cultures of tumor tissues confirm the DNA double-strand break repair defect of HPV-positive OPSCC and suggest a defect in the ATM-mediated DN... Background:HPV-positive head and neck squamous cell carcinoma of the oropharynx (OPSCC) are more sensitive towards radiation than HPV...
BD Difco Dehydrated Culture Media: MacConkey Agar Fisher Scientific DF0075-17-1 NanoDrop 1000 Spectrophotometer Thermo Scientific Uded to measure RNA concentration UV/Vis Spectrophotometer BECKMAN DU 530 Used to determine E. coli count iScript RT Supermix, 100 rxns Bio-Rad 1708841 iTa...
该方法提供了一个简单,重复性的方式,培养组织切片。它有很大的潜力,因为它允许成各种人体组织,其中包括恶性组织成完整的人体组织的基因传递的研究,基因传递的分析和关于尊重个人的患者之间的变异程度高的影响,可以提供重要的信息基因的吸收。成功的转基因表达在细胞的不同阶段的疗效,不同的癌症类型可能有不同...