The precursors of neutrophils and monocytes, granulocyte/macrophage colony-forming cells (GM-CFC) were elevated in the spleen, bone marrow, and peripheral blood. RIII-T3-conditioned medium stimulated bone marrow GM-CFC and caused the myelomonocytic cell line, WEHI-3B, to differentiate in vitro. ...
The resulting hybridomas were cultured in DMEM containing 15% FBS, 100 μM hyposiantine, 0.4 μM aminopterin, 16 μM thymidine (HAT; Sigma), and 50 pg/ml of murine IL-6 for 2 weeks. Conditioned medium of the hybridoma clones was checked for reactivity to CXADR ...
Elevated expression of POSTN in SCLC is correlated with accelerated tumor progression and metastasis. Conditioned medium rich in POSTN derived from SCLC tumors demonstrates the ability to activate HSCs in the liver microenvironment. Mechanistically, POSTN emerges as a binding partner for the membrane rece...
HUVECs were pre-incubated with the supernatant conditioned medium (CM) of A549 cells for 48 h before co-culturing with PBMC. PBMC were extracted from normal blood of healthy volunteers and treated with 2.5 µg/ml anti-CD3 (OKT3, eBioscience) antibody and IL-2 (100 U/ml) in order...
Co-culture of tumor cells with hASCs or the addition of conditioned medium (CM) from hASCs effected an increase in the proliferation of H460 or U87MG cells. Co-injection of hASCs with tumor cells effected an increase in tumor cell viability in vivo, and also induced a reduction in ...
Forms of cathepsin B greater in size and stability than the mature, lysosomal form have been observed in tumors from humans and animals or in media conditioned by them (10, 11, 12, 13). At present, many of these findings are unexplained. Normal human tissues and human tumors contain ...
Exosomes were isolated and purified from CRC cell culture-conditioned medium (CM) or CRC patient serum using ultracentrifugation, following a previously established exosome extraction method [4]. The quantification of exosomal protein was conducted using the BCA Protein Assay Kit (Beyotime Biotechnology,...
Ex vivo omental-tumor model The whole omentum was incised from SCID mice and rinsed with PBS. The murine omentum was then placed in a 6-well plate and pretreated with conditioned or normal medium containing 60 ng/mL GROα (Abnova). After 24 h, the omentum was refreshed with a condit...
Conditioned medium from endothelial cell cultures can restore the normal phenotypic expression of vascular endothelium maintained in vitro in the absence o... Bovine vascular endothelial cells continuously maintained and grown in the presence of FGF adopt at confluence the configuration of a cell monolaye...
22 Others have shown that, in vitro, metastatic melanoma expressing CCR7 have increased migration toward CCL21 containing conditioned medium from lymphatic endothelial cells.23 Our findings add to these observations by showing CCR7-expressing melanoma cells increase lymphatic density within (and surrounding...