Frank J. StroblJon E. LevineMethods in NeurosciencesFrank J. Strobl , Jon E. Levine . 1990. In Vivo Tissue Culture: Use of Ectopic Anterior Pituitary Gland to Study Neuropeptide and Steroid Actions on Adenohypophysis. Cell Culture, 316-329. /...
The primary objective of our humanized mouse experiments was to assess Cas9-EDVs for their ability to mediate cell-targeted genome editing and transgene delivery in vivo. Given that human CD19+B cells, in addition to T cells, engrafted in the second mouse cohort, we additionally assessed ...
Incubation in tissue culture media allows isolated rabbit proximal tubules to regain in-vivo-like transport function: response of HCO 3 − absorption to ... Kunimi,M; Muiler-Berger,S; Hara,... - 《Pflügers Archiv European Journal of Physiology》 被引量: 44发表: 2000年 Incubation in tissu...
S., Engstrom, A., Groen, S. S., Karsdal, M. A., Bay-Jensen, A. C. An Ex Vivo Tissue Culture Model of Cartilage Remodeling in Bovine Knee Explants. J. Vis. Exp. (153), e59467, doi:10.3791/59467 (2019). Download Citation Reprints and Permissions JoVE Privacy ...
We explored the feasibility of developing corporal tissue, consisting of human cavernosal smooth muscle and endothelial cells in vivo, using three-dimensional acellular collagen matrices, which are similar in architecture to native corpora. Acellular collagen matrices were derived from processed donor rabbit...
Colony assay using V79 cells, the agar diffusion assay with L929 cells, and the 7-day rabbit muscle implantation test were employed to evaluate the cytotoxicity and tissue toxicity of natural rubber latex (NRL) materials. The in vivo implantation test showed that, among 13 histological parameters...
. Tissue culture dishes were pre-coated with 0.2% gelatin in phosphate-buffered saline for MVECs, HRECs, and HUVECs35. All cells were cultured at 37 °C in a humidified 5% CO2atmosphere36. DNA constructs The 20 nt target DNA sequence (5′-GTCCCGGTACGAGCACTTGT-3′, mK22) ...
Such patients could benefit from transplantation of tissue-engineered vascular grafts using autologous cells. One challenge that has limited this development is the need for cell isolation, and risks associated with ex vivo expanded stem cells. Here we demonstrate a novel approach to generate ...
In spite of the advances in the knowledge of adipose-derived stem cells (ASCs), in situ location of ASCs and the niche component of adipose tissue (AT) remain controversial due to the lack of an appropriate culture system. Here we describe a fibrin matrix-supported three-dimensional (3D) ...
The intact brain and spinal cord tissue morphology (Fig. 9) suggest that Mab 5H7 confers effective passive protection against EV71 infection in vivo. Figure 9: Immunohistochemistry and histopathology of EV71-infected AG129 mice without and with therapeutic protection with 5H7. Brain (A–D) and ...