Cell-free DNA (cfDNA) quantitation in patients’ plasma has been recently applied in monitoring several cancer types. This study evaluates the diagnostic potential of cfDNA in PDAC patients.MethodsPlasma cfDNA levels and integrity ratio were assayed using quantitative real-time PCR of Alu-repeat ...
Cell-free DNA (cfDNA) quantitation in patients’ plasma has been recently applied in monitoring several cancer types. This study evaluates the diagnostic potential of cfDNA in PDAC patients.MethodsPlasma cfDNA levels and integrity ratio were assayed using quantitative real-time PCR of Alu-repeat ...
Evaluation of cell viability, mixed function oxidase activity, metallothionein induction, and genotoxicity in rainbow trout hepatocytes exposed to industri... The DNA damage induced in a human breast cancer cell line treated with 1,5 (10)-estradiene-3,4,17-trione (3,4-estrone-o-quinone; 3,4...
Combined microfluidic enrichment and staining workflow for single-cell analysis of circulating tumor cells in metastatic prostate cancer patients Article Open access 30 July 2024 Limitations and opportunities of technologies for the analysis of cell-free DNA in cancer diagnostics Article 31 January 202...
Calf diarrhea is associated with enteric infections, and also provokes the overuse of antibiotics. Therefore, proper treatment of diarrhea represents a therapeutic challenge in livestock production and public health concerns. Here, we describe the abilit
A decrease of the filipin concentration to 1 μg/ml did not improve cell viability significantly (82.4 ± 5.2% dead cells), thus filipin could not be used as an endocytosis inhibitor due to the significant combined toxicity with the P/G/L nanoparticle. However, genistein, another inhibitor ...
All production strains and both recipient hosts have been analyzed bywhole genome sequencing. This confirmed integrity of the expression cassettes and correct integration and deletion of genes, the absence ofplasmid DNAand the absence of antibiotic selection markers used during strain construction. ...
(about 1.3 kb), the hybridisation signal strength on chromosomes was weak and the frequency of hybridisation signals per cell was also low. Only about 30% metaphase cells had signals and signals were usually on only one chromosome, which is usually common in FISH mapping of cDNA clones [50]...
Preclinical data have shown that paclitaxel intensifies the cell-killing effects of chemically induced DNA damage by alkylating agents and cisplatin, provided that paclitaxel precedes these agents (Parker et al, 1993, Liebmann et al, 1994). In the clinical setting, paclitaxel has shown enhanced activ...
Metastatic non-small cell lung cancer (NSCLC) is a highly fatal and immunogenic malignancy. Although the immune system is known to recognize these tumor cells, one mechanism by which NSCLC can evade the immune system is via overexpression of programmed c