The epidermal growth factor receptor (EGF-R), unlike the EPO-R, is efficiently transported to the cell surface and displays a much longer metabolic half-life. To determine which EPO-R cytosolic domains are involved in intracellular degradation, we studied chimeric receptor molecules constructed of ...
Erythropoietin and epoetin alfa are cleared via uptake and degradation via the EPO-R-expressing cells, and may also involve other cellular pathways in the interstitium, probably via cells in the reticuloendothelial scavenging pathway or lymphatic system 4. Only a small amount of unchanged epoetin al...
, 1995). Binding at Y401 CIS1 inhibits STAT5 signaling and may promote erythropoietin receptor degradation (Matsumoto et al., 1997; Verdier et al., 1998). After phosphorylation of Y343 the Pim1 kinase is activated (Miura et al., 1994a), and this may attenuate apoptosis after cytokine ...
Human erythropoietin receptor: cloning, expression, and biologic characterization. Blood 1990;76:31–35. CAS PubMed Google Scholar Winkelmann JC, Penny LA, Deaven LL, et al. The gene for the human erythropoietin receptor: analysis of the coding sequence and assignment to chromosome 19p. Blood ...
Using electrophoretic mobility shift assays, we could demonstrate strong activation of NF-B by erythropoietin-receptor signaling in HeLa cells. Activation of NF-B did not require degradation of IBand was not prevented by proteasome inhibition. Furthermore, stimulation with erythropoietin resulted in a ...
A careful gathering of Epo/EpoR biomolecular information enabled us to assemble an unexpected jigsaw puzzle which, via distinct JAK-dependent and JAK-independent mechanisms and different internalization/recycling as well as ubiquitination/degradation pathways, could explain most of the controversies of ...
Hypoxia abrogates the degradation of hypoxia-inducible factors (HIF)-1 and -2, that can then bind to the hypoxia response element within the Epo gene, activating its transcription. Receptors for Epo are expressed on cells known to synthesize Epo, but also on cardiomyocytes, cardiac fibroblasts, ...
EPO is cytoprotective through the prevention of programmed cell death in both vascular and neuronal systems by modulating two distinct components of programmed cell death that involve the degradation of genomic DNA and the externalization of cellular membrane phosphatidylserine (PS) residues. Cytoprotection...
Clearance, however, appears to have a stronger influence on in vivo activity than receptor-binding affinity. Table 1 Animal studies of EPO in AKI: ischemia-reperfusion models Full size table Each EPO molecule has two EPO receptor (EPOR) binding sites. There are two affinities of the EPOR for...
The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. ...