elegans RNAi Collection (Ahringer) or the C. elegans ORF-RNAi Resource (Vidal)57, both distributed by Source BioScience. All clones were confirmed to contain the right gene by sequencing. All double knockdowns were performed with a single RNAi vector for high efficiency. In general, overnight...
While membrane insertion of single-spanning membrane proteins into the endoplasmic reticulum (ER) is relatively well understood, it is unclear how multi-spanning proteins integrate. We have investigated the cotranslational ER integration of a double-spanning protein that is derived from leader peptidase....
While membrane insertion of single-spanning membrane proteins into the endoplasmic reticulum (ER) is relatively well understood, it is unclear how multi-spanning proteins integrate. We have investigated the cotranslational ER integration of a double-spanning protein that is derived from leader peptidase....
Stevens et al., 1997); and (ii) using the non-random distribution of single and double substituted N2O isotopologues (Bergsma et al., 2001; Spott et al., 2006) to calculate both the fraction of pool-derived N2O and the 15N enrichment of the labelled pool undergoing N2O production (ap)...
Venlafaxine extended-release tablets should be administered in a single dose with food either in the morning or in the evening at approximately the same time each day. Each tablet should be swallowed whole with fluid and not divided, crushed, chewed, or placed in water. 2.1 Initial Treatment ...
At steady state the average bioavailability of metoprolol following administration of metoprolol succinate extended-release, across the dosage range of 50 to 400 mg once daily, was 77% relative to the corresponding single or divided doses of conventional metoprolol. The bioavailability of metoprolol ...
ultimately achieving the myelination of the recipient neuraxis only after a protracted period of postnatal maturation; this may reflect the incremental engagement of local axons by single oligodendrocytes, as the latter mature and expand their individual domains of myelin ensheathment, adding axons to ...
Long-lived proteins and dysfunctional organelles are thereby engulfed in newly formed double-membrane vesicles called autophagosomes, which eventually will undergo fusion with lysosomes. In the resulting autolysosomes, the autophagosomes and their cargo are degraded and the components recycled [4]. A ...
Macro-ER-phagy is characterized by the recruitment of double-membrane autophagosomes and requires both LC3 lipidation and autophagosome biogenesis machineries [2,135]. A recent study showed that procollagen is misfolding prone and resistant to ERAD-mediated degradation. Instead, procollagen is ...
cell is highest closer to the center, where the nucleus tends to be. As in all organelles, the membrane surrounding the many folds of the rough ER is a double plasma membrane; the ribosomes are attached to the outer portion of this membrane, that is, the side facing the cell cytoplasm....