EpCAM反义寡核苷酸1H8/CD3成功地抑制了肝癌移植瘤的生长,并降低了大多数CSC生物标志物的表达。为了降低T细胞活化引起的有害细胞因子毒性,开发了靶向NK细胞上的CD16和肿瘤细胞上的EPCAM的双特异性NK细胞活化子(Bike),以此介导ADCC对EPCAM+HT-29结肠癌细胞的杀伤作用。经白介素15交联剂修饰的三特异性NK细胞激活剂(...
Combination Therapy with EpCAM-CAR-NK-92 Cells and Regorafenib against Human Colorectal Cancer ModelsThe DNA sequence of the EpCAM-CAR was synthesized by GenScript (Nanjing, China). Then, the DNA fragment was inserted into a lentiviral vector-designated pRRL-GFP to generate the pRRL-EpCAM-CAR...
人源化epcam car可以与靶向其它肿瘤抗原或肿瘤微环境的car(例如vegfr ‑1‑ 3、pdl ‑ 1)组合。生成具有epcam和cd3或其它抗原的双特异性抗体用于治疗。 [0055] 人源化epcam ‑ car可以用于生成其它类型的细胞,诸如car ‑ 天然杀伤(nk)细胞、epcam ‑ car ‑ 巨噬细胞和epcam ‑ car造血细胞,...
靶向EPCAM和ICAM-1的双嵌合抗原受体.pdf,本发明涉及低亲和力双嵌合抗原受体(CAR),这些双CAR提供了针对异质性肿瘤的细胞毒性并减轻了中靶脱瘤毒性。这些双CAR被构建成具有两个结合结构域,这两个结合结构域具有50nM至50μM的降低的亲和力,其中一个结合结构域是淋巴细胞功能
向嵌合抗原受体(car)中的整合[0173]在某些实例中,本公开的epcam结合蛋白可以并入嵌合抗原受体(car)或procar中。工程化的免疫效应细胞,例如t细胞或nk细胞,可以用来表达car,该car包括含有例如本文所述的抗epcam单结构域抗体的抗epcam结合蛋白。在一个实施方案中,包括本文所述的epcam结合蛋白的car经由铰链区连接至跨...
本研究表明,这种无偏向性的分子基因与药物筛选策略,可以有效应用于以T细胞或者NK细胞为基础的细胞治疗设计中。本研究的方法与miR200c-Zeb1-EpCAM通路应用于过继细胞疗法申请了美国专利。本研究的第一作者,张明刚博士及团队近期在美国创立了细...
EpCAM-NK cells EpCAM-macrophages can be used for targeting different types of cancer EpCAM-CAR-T cells can be delivered intra-tumorally to patients for increased safety. The following examples further illustrate the present invention. These examples are intended merely to be illustrative of the ...
March 1st 2025 Article The FDA granted RMAT designation to nogapendekin alfa and CAR-NK for reversing lymphopenia in patients with cancer, with promising survival data in metastatic pancreatic cancer and non–small cell lung cancer. Read More...
Corrigendum to "Combination Therapy with EpCAM-CAR-NK-92 Cells and Regorafenib against Human Colorectal Cancer Models". [This corrects the article DOI: 10.1155/2018/4263520.]. Qing,Zhang,Haixu,... - 《Journal of Immunology Research》 被引量: 4发表: 2019年 加载更多站...
Previously, we constructed a bispecific NK-cell-engager (BiKE) bearing single-chain variable fragments (scFv) against CD16 on NK cells and EpCAM on tumor cells. This BiKE facilitated antigen-specific antibody-dependent cell-mediated cyto... Schmohl, J.U.,M Felices,L Taras,... - 《Molecular...