酶联免疫斑点技术(Enzyme-linked Immunospot Assay,ELISpot),是一种结合了细胞培养技术与酶联免疫吸附技术(ELISA)的高度敏感的免疫检测技术。该技术能够在单细胞水平检测细胞因子或免疫球蛋白的分泌情况,通过捕获培养中细胞分泌的细胞因子,并以酶联斑点显色的方式将其呈现出来。具有较高的特异性、直观可信度高,并且易操...
多因子 ELISPOT(Multicolor ELISPOT)技术 • 近年来,多因子 ELISPOT(Multicolor ELISPOT) 技术被开发出来,可以同时检测多个细胞因子在同一个样本中的分泌情况。这种技术能够更有效地评估疫苗引发的综合免疫反应。 • 例如,使用IFN-γ和 IL-2的双因子 ELISPOT板,研究者可以在同一孔板中同时检测两种细胞因子的分泌,提...
Synonyms enzyme-linked immunospot assay, ELISPOT; reverse enzyme-linked immunospot assay, RELISPOT Short Description The ELISPOT assay is a versatile test system for the quantitative assessment of the function of B cells, T cells and monocytes. Both drug-related immunotoxicity due to immunosuppression ...
酶联免疫斑点技术(Enzyme Linked Immunospot Assay,ELlSPOT)是细胞免疫学研究中最敏感的检测方法之一,可以在单细做水平对抗体分泌细胞(ASC)及细胞因子(CK)分泌细胞进行检测。由于是单细胞水平检测,ELISPOT比ELISA和有限稀释法等具有更高的灵敏性。能从20万-30万细胞中检出1个分泌该蛋白的细胞。并且该方法能够对抗原刺...
In the enzyme-linked immunospot (ELISPOT) assay, applied to anti-tumor T cells by Wölfel and co-workers[17,18•], antibodies are used to detect cytokines produced by individual cells after a short (6–48hour) period of re-stimulation with the antigen, usually a peptide. The definition...
Collection of 2ml heparinized blood for enzyme linked immunospot assay (ELISpot) was done. All patients received four antituberculous drugs, isoniazid, rifampicin, pyrazinamide and ethambutol, for the initial 2months. After 2months of therapy; another three successive sputum samples for sputum smear ...
Options for indirect measure of mucosal immune responses are generally more practical and these include the widely used enzyme-linked immunospot (ELISPOT) and the newer antibody in lymphocyte supernatant (ALS) assays [8]. Antibody secreting cells (ASCs) stimulated at one mucosal site transit through...
A modified enzyme-linked immunospot (ELISPOT) method was established and had been used for the detection of mouse IgG secretion in the anti-human CD8 hybridoma cells and the LACA mouse spleen cells. This method had been utilized to study the effect of "Liu Wei Di Huang" polysaccharide CA4-3...
We hypothesized that detection of activated mumps-specific antibody-secreting B cells (ASCs) by enzyme-linked immunospot (ELISPOT) assay could be used as a more reliable diagnostic. To test this, a time course of virus-specific ASC responses was measured by ELISPOT assay following MMR vaccination...
酶联免疫斑点实验(ELISPOT)2) Enzyme linked immunospot ELISPOT(酶联免疫斑点)3) Enzyme linked-immunospot assay 酶联免疫斑点法(ELISPOT)4) Enzyme-Linked Immunospot 酶联免疫斑点实验5) Dot-ELISA 斑点-酶联免疫吸附试验6) Dot-ELISA 斑点酶联免疫吸附试验 1. Checking Salmonella in Animal Feed by ...