T-cell responseT-cell frequencycytokine productionThe ELISPOT assay is widely used for monitoring of immune responses to anti-tumor vaccines. It is a versatile, robust assay, which can be formatted to measure the frequency of antigen-specific T cells in peripheral blood or in culture and to ...
Cox RJ. Early Induction of Cross-Reactive CD8+ T-Cell Responses in Tonsils After Live-Attenuated Influenza Vaccination in Children. J Infect Dis. 2020 Apr 7;221(9):1528-1537. doi: 10.1093/infdis/jiz583. PMID: 32255493; PMCID: PMC7137893.[...
The ELISPOT assay has been established for the direct ex vivo quantification of peptide-reactive T lymphocytes from peripheral blood mononuclear cells (PBMC). In this report we studied the ELISPOT assay conditions for the detection of T cell responses against protein antigens including Tetanus toxoid...
酶联免疫斑点实验(Enzyme-Linked Immunospot Assay,ELISpot)是一种高灵敏度的,可在单细胞水平检测细胞因子或免疫球蛋白分泌频率的免疫学检测手段;灵敏度可高达百万分之一。ELISpot现在被大量应用于疫苗研发、特异性免疫感染、癌症、过敏和自身免疫性疾病...
Yang, F., Patton, K., Kasprzyk, T., Long, B., Gupta, S., Zoog, S. J., Tracy, K., & Vettermann, C. (2022). Validation of an IFN-gamma ELISpot assay to measure cellular immune responses against viral antigens in non-human primates. Gene therapy, 29(1-2), 41–54. https:/...
ELISPOT assay for measurement of antigen-specific and polyclonal antibody responses. In: Coligan J, et al., editors. Current Protocols in Immunology. Hoboken, NJ: John Wiley and Sons Inc; 1996. pp. 7.14.1–7.14.7.Lycke NY and Coico RF (1996). ELISPOT assay for measurement of antigen- ...
The enzyme-linked immunospot (ELISPOT) assay is a highly sensitive and reproducible method for quantifying T cell-mediated immune responses, and has been used to measure antigen-specific responses post-vaccination. While there are several advantages of the ELISPOT assay for use in field settings for...
目前,临床上应用的结核感染T细胞免疫检测的IGRAs技术有两种:一是ELISPOT;一是酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)。IGRA-ELISPOT方法(也称为细胞检测)采用的ELISPOT技术是细胞培养技术与ELISA技术结合产生的新 2020-01-03 21:39 News WIKI 相关搜索 ELISPOT...
Assessment of donor-specific alloreactive memory/effector T cell responses using an IFN-γ Elispot assay has been suggested to be a novel immune-monitoring tool for evaluating the cellular immune risk in renal transplantation. Here, we report the cross-validation data of the IFN-γ Elispot assay ...
One assay, the enzyme-linked immunospot (ELISpot) [T-SPOT.TB; Oxford Immunotec; Oxford, UK] enumerates IFN-γ–secreting T cells, while the other ... A Lalvani - 《Chest》 被引量: 1007发表: 2007年 Cellular and humoral immune responses induced by intradermal or intramuscular vaccination with...