目前常用于检测抗原特异性T细胞的主要是以下方法:应用肽-MHC多聚体直接标记抗原特异性TCRs以及功能分析(包括胞内细胞因子检测,ELISPOT,细胞因子捕获技术,或者活化指标的上调检测)。 由于抗原特异性T细胞数量稀少,为了快速、简便、有效地检测它们,BioLegend...
Enzyme-linked immune absorbent spot (Elispot) is a quantitative method for measuring relevant parameters of T-cell activation. The sensitivity of Elispot allows ...
ELISPOT T-Cell Response to Measles and Varicella-Zoster Antigen in Adults, Children, and HIV-Infected ChildrenL. L. Yasukawa
还提供特色的应用于模式动物斑马鱼的抗体。此外还提供ELISA试剂盒,ELISPot试剂盒,FluoroSpot试剂盒等。应用类型 WB 免疫原 该T-Cell Cytokine Receptor(TCCR)(C-Terminus)antibody(T-Cell Cytokine Receptor (TCCR) (C-Terminus) antibody)产品的免疫原详情,请查看Covalab提供的产品说明书。
2009. Quantifying HIV-1-specific CD8(+) T-cell responses using ELISPOT and cytokine flow cytometry. Methods Mol. Biol. 485 : 359-374... BL Shacklett,JW Critchfield,D Lemongello - 《Methods in Molecular Biology》 被引量: 14发表: 2009年 Modified vaccinia Ankara-expressing Ag85A, a novel tu...
A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells. J. Immunol. Methods 65, 109–121 (1983). Article CAS PubMed Google Scholar Draenert, R. et al. Comparison of overlapping peptide sets for detection of antiviral CD8 and CD4 T cell ...
In a second group of 40 patients with COPD and smoking controls, T-cell responses against extracellular matrix (elastin, collagen I and collagen V) were determined with enzyme-linked immunosorbent spot (EliSpot) (interferon γ (IFNγ) and interleukin-2) on peripheral blood mononuclear cells and ...
ELISPOT平板准备过程如下:每孔加入35%酒精50微升以预湿润板底,以每块平板10毫升无菌PBS稀释100微升抗-IFNγ捕捉抗体。然后将100微升的稀释捕捉抗体等份加入各孔。4℃下温育平板过夜。温育后,洗涤平板(程序1,平板类型2,96孔板洗涤仪;BioTech)以除去捕捉抗体。然后将含有10%血清的1640培养基以100μl/孔加入各孔并在...
In this Phase IIb clinical trial, up to 75% of volunteers immunized with an rADV‑5 vector encoding HIV Gag–Pol and Nef (negative factor) had IFNγ-producing cells, as detected by elispot analysis. Unfortunately, the clinical trial was halted before its completion after the first ...
The frequency of cytokine-producing, indirectly primedalloreactive T cellsdetermined by enzyme-linked immunospot (ELISPOT) analysis was further refined by the Harvard group usingin vitrodepletion of putative CD25+Treg[60]. In a series of renal transplant recipients, incubation of PBMC with anti-CD25 ...