进一步实验团队想确定肺中是否存在这样一群细胞,便在ELISpot版上培养了未刺激的EV肺细胞。发现只有在感染组中存在这样一群B细胞。具有CD138+CD38lo表型的EV浆细胞在感染组小鼠中富集,同时这群细胞还表达Blimp-1 marker。而CD38+CD138-(同时表达B220和CD19)的这群细胞被认为是LLPCs的前体细胞。支气管肺泡灌洗液(B...
While the design is similar to an ELISA, ELISpots quantify the number and relative amount of secreted protein on a single cell level, as opposed to an ELISA that reveals the concentration of secreted proteins from a population of cells. The sensitivity, robustness, and diversity of different ...
2.2. Memory B Cell ELISpot After 5 days of culture, the cells were transferred to prepared 96-well Multiscreen IP filter plates (Millipore, Merck KGaA, Darmstadt, Germany) and cultured again. The plates were first washed with 35% ethanol and PBS and then coated with 100 µL antigen or ...
In human subjects CD27 expression is often used as a marker of the memory B-cell phenotype. Three types of CD27+memory B cells have been identified in the circulation: IgM+IgD−CD27+IgM-producing memory B cells, IgM−IgD−CD27+class-switched memory B cells, and IgM+IgD+CD27+margi...
鼠标记忆B细胞刺激物又称Mouse memory B-cell stimpack,是由Mabtech品牌生产的高品质产品,相对于同类产品具有极高的性价比。详细背景资料欢迎查看Mabtech品牌提供的英文产品说明书。 产品描述 该鼠标记忆B细胞刺激物产品的详细产品描述欢迎查看品牌提供的英文产品说明书。
and t-cell subsets is described in the supplementary materials and methods . 2.4. cell cultures for polyclonal stimulation of memory b cells 1–2×10 6 pbmcs were cultured for 5 days with cpg as described before [27] . 2.5. elispot elispot for total and pneumococcal polysaccharide specific ...
3 m, 9 m and 12 m compared to AC (Fig.6g,h). As an additional support, we analyzed B cell subsets from 3 y post-infection individuals who showed positive DBL-TH-specific MBCs by ELISPOT. We found that the frequencies of activated and atypical MBCs were significantly high ...
The donor-specific memory B cell ELISpot can be used in HLA diagnostic laboratories as a cross-match assay to quantify donor-specific memory B cells in patients with a history of sensitizing events. 展开 关键词: basic (laboratory) research/science clinical research/practice immunobiology organ ...
stronger T cell responses, including IFN-γ-positive and proliferating T cells, than low responders at 28 days pso (Fig.7a). Antibody responses to spike protein from 229e and NL63, B cell ELISpot and ADNKA were increased in low responders early after SARS-CoV-2 infection in comparison to...
Furthermore, we quantified the Ro- and La-specific memory B cells in these individuals by a 6-day in vitro polyclonal stimulation of PBMC followed by an antigen-specific ELISPOT assay for the detection of memory B cells. In addition to this, ELISA profiling of autoantibodies was carried out ...