We then used a more sophisticated approach to detect fine changes occurring within different subpopulations of CD4+T cells. For each patient and control, data from 5000 CD45+CD3+CD4+T cells were exported and concatenated in a unique matrix. We explored the T helper cell panel by unsupervised ...
Activation induced apoptosis in lymphocytes [J]. Curr Opin Immunol, 1994, 6(3): 476-487. [4] Okamoto Y, Abe T , Niwa T, et al. Development of a dual color enzymelinked immunospot assay for simultaneous DOC格式论文,方便您的复制修改删减 detection of murine T helper type 1 and T helper...
T-cell apoptosis assay PBMCs were isolated from healthy human donors using Lymphoprep density gradient centrifugation. PBMCs were plated at a density of 2 × 106per well in six-well plates and stimulated with Skov3 (CP) cell lysate, anti-CD3e (10 μg ml−1) and anti-CD28 (2 ...
别名: TUNEL细胞凋亡检测试剂盒,TUNEL Assay Apoptosis Detection Kit 产地: 上海 品牌: 爱必信(absin) 分子式: - 包装规格: 20T;50T 货号: abs50047 是否进口: 否 产品描述: 细胞发生凋亡时, 会激活一些 DNA 内切酶,这些内切酶会切断核小体间的基因组 DNA,产生 180 bp-200 bp 的 DNA 片段,表现...
上海康朗生物科技有限公司生产的TUNEL细胞凋亡检测试剂盒,最新报价:2000元/T,TUNEL细胞凋亡检测试剂盒▼▲英文名称TUNEL中文名称TUNEL细胞凋亡检测试剂盒别名TUNELApoptosisAssayKit;InSituCellDe
ROR gamma, a NR1 Thyroid Hormone-Like receptor, has been shown to affect thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. ROR gamma has been shown to promote thymocyte survival by activating the expression of the antiapoptotic protein Bcl-x(L). ROR gam...
ROR gamma, a NR1 Thyroid Hormone-Like receptor, has been shown to affect thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. ROR gamma has been shown to promote thymocyte survival by activating the expression of the antiapoptotic protein Bcl-x(L). ROR gamma is ...
In vitro primary human lymphocyte flow cytometry based micronucleus assay: simultaneous assessment of cell proliferation, apoptosis and MN frequency. In order to minimise the number of positive in vitro cytogenetic results which are not confirmed in rodent carcinogenicity tests, biological systems that a...
Cell Apoptosis Biochemical Assays DNA fragmentation represents a characteristic of late stage apoptosis. DNA fragmentation in apoptotic cells can be detected by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL). The TUNEL assay relies on the presence of nicks in the DN...
(Fig. 1b). The cell growth could be readily detected by flow-cytometry (Fig. 1c). The hallmark for cell cycle entry is the DNA synthesis and subsequent cell division. Using BrdU incorporation assay, we found that while the DNA synthesis of non-activated naive T cells was virtually ...