酶联免疫斑点技术(Enzyme-linked Immunospot Assay,ELISpot),是一种结合了细胞培养技术与酶联免疫吸附技术(ELISA)的高度敏感的免疫检测技术。该技术能够在单细胞水平检测细胞因子或免疫球蛋白的分泌情况,通过捕获培养中细胞分泌的细胞因子,并以酶联斑点显色的方式将其呈现出来。具有较高的特异性、直观可信度高,并且易操...
Czerkinsky, Enzyme-linked immunospot assays for direct ex vivo measurement of vaccine-induced human humoral immune responses in blood, Nature Protocols 8 (2013) 1073-1087.Saletti, G.; Ç uburu, N.; Yang, J. S.; Dey, A.; Czerkinsky, C. Enzyme-Linked Immunospot Assays for Direct ...
网络酶联免疫斑点 网络释义 1. 酶联免疫斑点 用酶联免疫斑点(Enzyme-linked immunospot)和有限稀释检测也能获得相似的结果。在体外用CFSE标记的PBMC再刺激结合… www.wibp.com.cn|基于 1 个网页 释义: 全部,酶联免疫斑点
酶联免疫斑点技术(Enzyme Linked Immunospot Assay,ELlSPOT)是细胞免疫学研究中最敏感的检测方法之一,可以在单细做水平对抗体分泌细胞(ASC)及细胞因子(CK)分泌细胞进行检测。由于是单细胞水平检测,ELISPOT比ELISA和有限稀释法等具有更高的灵敏性。能从20万-30万细胞中检出1个分泌该蛋白的细胞。并且该方法能够对抗原刺...
网络释义 1. 酶联免疫斑点检测 酶联免疫斑点检测(Enzyme-Linked Immunospot Assay) 免疫酶技术(Immunoenzyme Techniques) 分枝杆菌结核(Mycobacteriu… new.med.wanfangdata.com.cn|基于3个网页 2. 酶联免疫斑点技术 酶联免疫斑点技术... ... ) enzyme linked immunospot assay 酶联免疫斑点法 )Enzyme-linked imm...
In the enzyme-linked immunospot (ELISPOT) assay, applied to anti-tumor T cells by Wölfel and co-workers[17,18•], antibodies are used to detect cytokines produced by individual cells after a short (6–48hour) period of re-stimulation with the antigen, usually a peptide. The definition...
Enzyme-linked immunospot measurement of phosphoantigen-specific IFN-- 翻译结果3复制译文编辑译文朗读译文返回顶部 特定于 phosphoantigen 的干扰素酶联酶联免疫斑点测量-- 翻译结果4复制译文编辑译文朗读译文返回顶部 正在翻译,请等待... 翻译结果5复制译文编辑译文朗读译文返回顶部 phosphoantigen具体IFN- 的酵素连接...
2011. Enzyme-linked immunospot assay detection of mumps-specific antibody-secreting B cells as an alternative method of laboratory diagnosis. Clin. Vaccine Immunol. 18:35- 42. http://dx.doi.org/10.1128/CVI.00284-10.Enzyme-Linked ... Latner DR,McGrew M,N Williams,... 被引量: 28发表: 0...
Cultured enzyme-linked immunospot assay (ELISPOT) enhances detection of low HIV-specific immune responses in exposed seronegative individuals in Kenyaavian influenzavaccinationdisease controlThere is increasing evidence that many HIV exposed, seronegative individuals generate HIV-specific cellular immune ...
[目的]采用斑点酶联免疫吸附试验(Dot-ELISA)法检测系统性红斑狼疮(SLE)患者血清中抗鸡卵核42000蛋白的抗体,探讨该抗体与SLE患者相关抗体检测指标的关系,以进一步研究该抗体在SLE患者发病过程中的作用。 3. The McAb can also identify CEF infected by MDV through dot-ELISA. 1∶ 10 0稀释时与 GA株感染的 CE...