Enzymes are most commonly used to detect proteins via direct and indirect antibody detection strategies, where the enzyme is conjugated to either the primary antibody that binds the target protein or the secondary antibody that targets the primary antibody, resp...
Antibody-rest signal generator comprising an antibody covalently attached to a signal generating moiety by a heterobifunctional PEG linker, wherein the conjugate has the general formula: ** ** Formula wherein Ab is an antibody, SM is a moiety generator signal carrying an enzyme or a quantum ...
EnzymeAntibody enzyme conjugates Horseradish peroxidase (HRP) conjugates Select ELISA HRP-conjugated secondary antibodies Learn more about our HRP-Linked Secondary Antibodies Alkaline phosphatase (AP) conjugates Select ELISA AP-conjugated secondary antibodies Learn more about our Alkali...
网络抗体 网络释义 1. 抗体 例如在一个酶分子上修饰多 个抗体(Enzyme-conjugated antibody),预先在电极表面上固定一层抗原,靠抗原 -抗体的特异性 … www.docin.com|基于 1 个网页
First an unlabeled primary antibody, which is specific for the antigen, is applied. Next, an enzyme-linked secondary antibody which is reactive against the primary antibody is added. For enzymatic detection and production of a calorimetric signal, the appropriate enzyme substrate is added and this ...
For the cell surface marker CD133, single-cell suspensions in 100 μl assay buffer (phosphate buffered saline pH 7.2, 0.5% bovine serum albumin, 2 mM EDTA) were incubated with 10 μl of phycoerythrin (PE)-conjugated anti-CD133 antibody (clone 293C3, Miltenyi Biotec, Auburn, CA) for 10...
Detection is a two-step process. First,an unlabeled primary antibody binds to the specific antigen. Second, an enzyme conjugated secondary antibody that is directed against the host species of the primary antibody is applied. How do you read Elisa?
a secondary antibody conjugated with the enzyme is added, which finds the antigen. In this way, the antigen is sandwiched between two antibodies and the antigen is detectedby the change of substrate color in the presence of an enzyme (Zhao et al., 2014a). The most commonly used enzymes in...
The cells were then incubated with AlexaFluor 488/594/647 conjugated secondary antibodies for 1 h at RT. Imaging Slides were imaged using either a Leica microscope equipped with a × 63 objective and a CCD camera or a Nikon Eclipse Ti microscope equipped with a × 60/1.49 NA objective ...
Membranes were blocked in 5% BSA for 1 h, and followed by the appropriate antibody overnight at 4 °C and then incubated with horseradish peroxidase-conjugated secondary antibodies at room temperature for 1 h. Membranes were visualized with an enhanced chemoluminescence detection system. RNA...