SAM or enCRISPRa-mediated enhancer activation in HEK293T cells. mRNA expression relative to nontransduced cells is shown as mean ± SEM (n = 4 experiments). The differences between control and dCas9 activators were analyzed by a one-way ANOVA.#P < 0.05, ###P < 0....
Regulatory elements play a critical role in development process of eukaryotic organisms by controlling the spatio-temporal pattern of gene expression. Enhancer is one of these elements which contributes to the regulation of gene expression through chroma
red = 5kb resolution; green = 10kb resolution; violet = 25kb resolution. b unsupervised clustering of gene expression (rna-seq) of lal-b, naïve-b, mem-b, and bulk-b cells (triplicates for each category) obtained from calderon et al. nat. gen 2019. data were normalized...
These results raise the question of how enhancers affect gene expression in their native contexts. A simple interpretation is that enhancers act in a stochastic fashion to increase the probability that a regulated gene will be transcribed; such a model is compatible with programs of cell ...
Renin enhancer is critical for control of renin gene expression and cardiovascular function. J Biol Chem 281: 31753-31761, 2006.Adams DJ, Head GA, Markus MA, Lovicu FJ, van der Weyden L, Kontgen F, Arends MJ, Thiru S, Mayorov DN, Morris BJ. Renin enhancer is critical for control of...
HV Melchner,Frank Schnutgen,W Wurst,P Ruiz 被引量: 5 摘要: The present invention relates to a novel class of gene trap vector (enhanced gene trap vectors, eGTV) for efficiently identifying silent or weakly expressed target genes in mammalian genomes, methods of their production and methods ...
Enhancers play an important role in morphological evolution and speciation by controlling the spatiotemporal expression of genes. Previous efforts to understand the evolution of enhancers in primates have typically studied many enhancers at low resolutio
Thus, increased expression of the C/EBPβ and C/EBPδ proteins coincided with their enhanced DNA-binding activity in the lung. To localize the expression of C/EBPδ protein, we performed immunohistochemical staining in lung sections after LPS administration. We found strong staining for C/EBPδ...
a本实验克隆的ap2基因增强子和启动子的碱基组成与GenBank中的ap2基因序列完全一致,通过DNA重组技术将该基因增强子和启动子重组于pEGFP-N1真核表达载体上,成功构建了脂肪组织特异表达的pEGFP-N1-ap2重组质粒。为下一步转基因动物的研究奠定了实验基础。 This experimental clone ap2 gene increases the hadron and the...
(Illumina). A filtered joint digital gene expression and chromatin accessibility matrix was generated against the 10 × Genomics mm10-2020-A reference build (version 2020-A, Assembly: GRCm38, ENSEMBL release 98; Annotations: Gencode vM23) using a modified 10 × Genomics CellRanger-ARC...