In T cells, there are multiple examples of enhancer-specified gene regulation. Among the best characterized are cytokine genes, particularly theIfngandIl4-Il13cytokine loci where multiple enhancers and locus control regions have been identified3,4,5,6. Several of these elements, many based on cons...
📝Note:This is a revamp of the ABC codebase presented in [1]. If you wish to access that version of the ABC repo, please check outhttps://github.com/broadinstitute/ABC-Enhancer-Gene-Prediction/tree/master. We have documented the codebase and usage inRead The Docs ...
Nature (Lond - Parker - 1983 () Citation Context ...ific receptors which in turn modulate gene expression (for review, see reference 10). Data have recently accumulated on the binding of steroid-receptor complexes to regulatory regions of cloned genes =-=(34)-=-. However, little is known...
From the perspective of chromatin packaging, it is useful to cluster multiple TFBSs together, so that additive binding of the required TFs could progressively overcome the higher propensity of the enhancers to form nucleosomes, allowing desired gene regulation. From the perspective of cellular phenotyp...
Enhancer RNAscan be involved in regulation of gene expression via interactions with different proteins, such as theMediator complex, transcription factors,cohesins, or RPII. Similar tolncRNAs, eRNA may provide baits and scaffolds or even traps for their interactors. Some eRNAs may have more than on...
Regulation of IgH gene assembly: role of the intronic enhancer and 5'DQ52 region in targeting DHJH recombination. Regulation of Igh gene assembly: role of the intronic enhancer and 5'DQ52 region in targeting DHJH recombination.Afshar, R., Pierce, S., Bolland, D... R Afshar,S Pierce,...
A genome-wide framework for mapping gene regulation via cellular genetic screens Cell, 176 (2019), pp. 377-390.e19 View PDFView articleGoogle Scholar Goardon et al., 2006 N. Goardon, J.A. Lambert, P. Rodriguez, P. Nissaire, S. Herblot, P. Thibault, D. Dumenil, J. Strouboulis,...
However, we observed little or no effect on eRNA expression when we targeted gene promoters to drive mRNA expression (Figure 4D, Supplementary Figure S4), suggesting that enhancer regulation of linked mRNA is a unidirectional phenomenon. Moreover, we did not observe any effects of enhancer ...
The type 1 glucose transporter (GLUT1) gene encodes an integral membrane glycoprotein responsible for facilitating transfer of glucose across plasma membrane and is rapidly activated by serum, growth factors, and by oncogenic transformation. To elucidate the molecular mechanisms of regulation of GLUT1 ...
Enhancers are critical genomic elements that define cellular and functional identity through the spatial and temporal regulation of gene expression. Recent studies suggest that key genes regulating cell type-specific functions reside in enhancer-dense genomic regions (i.e., super enhancers, stretch enhanc...