The internal ribosomal entry site (IRES)from encephalomyocarditis virus (EMCV) is a popular RNA element used widely in experimental and pharmaceutical applications to express proteins in eukaryotic cells or cell-free extracts. Inclusion of the wild-type element in monocistronic or bicistronic messenger...
Here, using two tricistronic vectors designed for expression in mammalian hosts, we present evidence of EMCV IRES activity in prokaryotes. This finding enables the development of new and more versatile plasmid vectors for the production of recombinant proteins in multiple hosts from a single construct...
Urwin, P., Yi, L., Martin, H., Atkinson, H., and Gilmartin, P. M. (2000) Functional characterization of the EMCV IRES in plants. Plant J. 24 , 583–589.Urwin P., Yi L., Martin H., Atkinson H., Gilmartin P. M. (2000). Functional characterization of the EMCV IRES in ...
EMCV IRES supports cap-independent translation in axons. A-BAlmudena, Pacheco
Wagstaff M J,Lilley C E,Smith J,et al.Gene transfer using a disabled herpes virus vector containing the EMCV IRES allows multiple gene expression in vitro and in vivo[J].Gene Ther,1998,5(11):1566-1570Wagstaff et al. " Gene Transfer Using a Disabled Herpes Virus Vector Containing the ...
Wagstaff M J D,Lilley C E,Smith J,et al.Gene transfer using a disabled herpes virus vector containing the EMCV IRES allows multiple gene expression in vitro and in vivo. Gene Therapy . 1998Wagstaff MJ,Lilley CE,Smith J, et al.Gene transfer using a disabled herpes virus vector containing ...
462. Comparison of EMCV IRES and FMDV 2A Cleavage Factor for Expression of Canine MGMT and a Second Transgene Using HIV-1 VectorsWe previously described the cloning and expression of canine methylguanine-DNA methyl transferase (MGMT) using monocistronic HIV-1 and gamma-retroviral vectors (Zaboikin...
CHEMICAL COMMUNICATIONS- ROYAL SOCIETY OF CHEMISTRYChanK.RameshV.K.K. Chan, V. Ramesh, Conserved RNA motifs of EMCV IRES as potential building blocks to design RNA nanostruc- tures, Chem. Commun. (Camb.) 48 (2012) 11573-11575.
EMCV IRESGNRA tetraloopImino proton exchangeMg 2+NMRRAAA hairpin motifUsing NMR magnetization transfer from water and ammonia-catalyzed exchange of the imino protons, changes have been monitored in base-pair kinetics induced by Mg~(2+) in two key activity fragments r(CACCUGGCGACAGGUG) and r(...
Relative strength of EMCV IRES variants in CHO K1 cells in transient transfections.Esther Y. C. KohSteven C. L. HoZhiwei SongXuezhi BiMuriel BardorYuansheng Yang