Translational efficiency of EMCV IRES in bicistronic vectors is dependent upon IRES sequence and gene location. BioTechniques. 2006; 41 :283–284. 286, 288. passim. [ PubMed ]Bochkov YA, Palmenberg AC. 2006. Translational efficiency of EMCV IRES in bicistronic vectors is dependent upon IRES ...
“GCTAGA”;TheblackboxrepresentstheDsRedcodingsequence;TheblankboxrepresentstheEGFPcodingsequence;ThegrayboxrepresentstheEMCVIRESsequence;ThethinlinerepresentstheRestrictionEnzymecuttingsites2.2 EMCV 末端序列的增添严重影响其IRES 活性 本实验在构建质粒的过程中,插入了多个酶切位点,使得最终EMCVIRES 序列末端增添了“...
为研制脑心肌炎病毒(EMCV)新型活载体疫苗,首先将病毒核衣壳前体多肽P1与P12A3C串联基因分别插入穿梭质粒pDC316中,构建得到了重组穿梭质粒pDC316-P1和pDC316-P12A3C。3. A pair of primers was designed according to the sequence of the VP1 gene of porcine encephalomyocarditis virus(EMCV) and a recombinant pla...
Article: Three-color flow cytometry analysis of tricistronic expression of eBFP, eGFP, and eYFP using EMCV-IRES linkages
Translation initiation of encephalomyocarditis virus (EMCV) and foot and mouth disease virus (FMDV) occurs by a novel cap-independent mechanism which involves a RNA sequence termed internal ribosomal entry site (IRES). The role of IRES involves the recruit...
Methods:Bicistronic eBFP/eGFP and eBFP/eYFP constructs were made on two mammalian episomal plasmids using an internal ribosomal entry sequence from encephalomyocarditis virus (EMCV-IRES) to link two GFP expressions. Simultaneous two-color flow cytometry (FCM) analysis was accomplished using a dua...