This review is focused on whether modern (third generation), more rapidly degradable HES preparations with a lower M(w) (130 kD) and a lower MS (<0.5) are safer and have fewer side effects. Several studies demonstrated that such modern HES preparations appear to be safe with regard to ...
Embryonic stem cells Embryonic stem cells(ESCs) are pluripotent and typically derived from theinner cell massof the blastocyst-stage embryo. While ESCs are capable of self-renewing indefinitely while retaining their stemness, and generally do not undergo senescence in culture, there are several inhere...
Human embryonic stem cells(hESC) are self-renew unlimitedly and pluripotent,they have an ability to differentiate to multiple cell types representing all primitive embryonic germ layers: ectoderm,mesoderm and endoderm under appropriate conditions.These properties imply great potential in regenerative medicin...
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Here, we present evidence that the tumor-like growth of mouse embryonic stem cells (mESCs) is suppressed by short-term serum-free culture, which is reversed by pharmacological inhibition of Gsk3β. Mouse ESCs maintained under standard conditions using fetal bovine serum (FBS) were cultured in a...
200 Longwood Avenue, Boston, MA 02115, USA; 5Harvard Stem Cell Institute, Harvard Medical School, WAB-149G, 200 Longwood Avenue, Boston, MA 02115, USA Pluripotent stem cells, like embryonic stem cells (ESCs), have specialized epigenetic landscapes, which are impor- tant for pluripotency mainten...
Instead of citing nature as the grounds for demarcating moral status, perhaps it would be better to look at the decisions and activities that demarcate nature and establish the nature–culture gap. Our decisions and activities are expressions of our understanding of ourselves and I would like to...
Human embryonic stem cells: derivation, culture, and differentiation: a review. The greatest therapeutic promise of human embryonic stem cells (hESC) is to generate specialized cells to replace damaged tissue in patients suffering from... T Vazin,WJ Freed - 《Restorative Neurology & Neuroscience》...
Cell culture Mouse embryonic stem cells (mESCs) were cultured feeder-free in 0.1% gelatin-coated flasks (Sigma, G1890) under standard conditions (10% CO2, 5% O2, 90% humidity, 37 °C) in KnockOut DMEM (LifeTechnologies, 10829018), 2 mM Alanyl-glutamine (Sigma, G8541), 0.1 mM...
Concise review: The evolution of human pluripotent stem cell culture: from feeder cells to synthetic coatings. Current practices to maintain human pluripotent stem cells (hPSCs), which include induced pluripotent stem cells and embryonic stem cells, in an undifferen... LG Villa‐Diaz,AM Ross,J ...