Stem Cell Rev Rep 6:425-437.Yoon TM, Chang B, Kim HT, Jee JH, Kim DW, Hwang DY. Human embryonic stem cells (hESCs) cultured under distinctive feeder-free culture conditions display global gene expression patterns similar to hESCs from feeder- dependent culture conditions. Stem Cell Rev ...
Conditioning of human embryonic stem cell-derived endothelial cells with PBMCs confers TLR4 sensing in co-culture conditionsHuman embryonic stem cell-derived endothelial cells (hESC-EC) are being investigated as potential therapies for vascular disease. We have previously shown that hESC-EC do not ...
Although isolation and characterization of embryonic stem cells have been successful in cattle, maintenance of bovine embryonic stem cells in culture remains difficult. In this study, we compared different methods of cell passaging, feeder cell layers and medium conditions for bovine embryonic stem cell...
Embryonic stem cells(ESCs) are self-renewable, pluripotent cells derived from theinner cell massof a blastocyst-stage embryo.26These cells are characterized by nearly unlimited self-renewal, and during differentiation in vitro, they develop into cellular derivatives of endodermal, mesodermal, and ecto...
Both physical and chemical signals in the culture environment mediate stem cell fate. For research and the clinical application of stem cells, the development of defined and xeno-free culture conditions to maintain the undifferentiated growth of human embryonic stem cells (hESCs) is highly desirable....
Mouse embryonic stem (ES) cell culture. Mouse embryonic stem (ES) cells are used to generate mouse mutants by gene targeting and blastocyst-mediated transgenesis. ES cells must be cultured under conditions that prevent differentiation to maintain their ability to transmit alte... DA Conner - Curr...
The application of human embryonic stem cells (HESCs) to provide differentiated cells for regenerative medicine will require the continuous maintenance of the undifferentiated stem cells for long periods in culture. However, chromosomal stability during
involved in maintaining embryonic stem cells in the best possible state for their use in regenerative medicine. Their results, published inNature Communications, will help to find novel stem-cell therapies for brain stroke, heart disease or neurodegenerative conditions like Alzheimer's or Parkinson's ...
Oct3/4,Sox2,c-Myc,andKlf4,underEScellcultureconditions.Unexpectedly,Nanogwasdispensable.Thesecells,whichwedesignatediPS(inducedpluripotentstem)cells,exhibitthemorphologyandgrowthpropertiesofEScellsandexpressEScellmarkergenes.SubcutaneoustransplantationofiPScellsintonudemiceresultedintumorscontainingavarietyoftissuesfrom...
Embryonic stem (ES) cells are characterized by pluripotency, defined as the developmental potential to generatecell lineagesderived from all three primarygerm layers. In the past decade, great progress has been made on the cell culture conditions, transcription factor programs and intracellularsignaling ...