Moon SY,Park YB,Kim DS,et al.Generation, culture, and differentiation of human embryonic stem cells for therapeutic applications. Molecular Therapy . 2006Moon SY, YB Park, DS Kim, SK Oh and DW Kim. (2006). Gene
Mouse Embryonic Stem Cell (mESC) Culture Medium (100 mL of complete medium) To prepare 100 mL of complete mESC Culture Medium, aseptically mix the following components: ComponentVolume KnockOut™ DMEM...
Embryonic stem cells are totipotent stem cells derived from the inner cell mass of blastocysts. Under specific culture conditions, embryonic stem cells differentiate into multicellular embryoid bodies that contain differentiated cells from all three germ layers including cardiomyocytes. Human embryonic stem ...
Several applications require the maintenance of human embryonic stem cells (hESCs) in the absence of a murine embryonic fibroblast (MEF) feeder layer. This protocol describes the culture of hESCs on matrix-coated plates in the presence of MEF-conditioned media (MEF-CM)...
(2008). Indole derivatives sustain embryonic stem cell self-renewal in long-term culture. Biosci Biotechnol Biochem 72(5), 1242-8.Miyabayashi T, Yamamoto M, Sato A, et al. Indole derivatives sustain embryonic stem cell self-renewal in long-term culture. Biosci Biotechnol Biochem. 2008; 72...
Embryonic stem cells Embryonic stem cells(ESCs) are pluripotent and typically derived from theinner cell massof the blastocyst-stage embryo. While ESCs are capable of self-renewing indefinitely while retaining their stemness, and generally do not undergo senescence in culture, there are several inhere...
Xeno-free and serum-free culture systemChemically defined mediumHuman foreskin fibroblast cellsElimination of all animal components during derivation and long-term culture of human embryonic stem cells (hESCs) is necessary for future applications of hESCs in clinical cell therapy. In this study, we ...
Pluripotent stem cells express a variety of cell surface proteins which may be used for their characterization and isolation from heterogeneous populations under culture conditions. The combined use of pluripotency markers to identify ESCs facilitates downstream applications aimed at the propagation of ESCs...
Pluripotent stem cells, like embryonic stem cells (ESCs), have specialized epigenetic landscapes, which are important for pluripotency maintenance. Transcription factor-mediated generation of induced pluripotent stem cells (iPSCs) requires global change of somatic cell epigenetic status into an ESC-like ...
Mouse ESCs also contribute to a wide range of adult tissues in chimeras with intact embryos, including the germ cells. Recombinant Leukemia Inhibitory Factor (LIF) is a bioactive protein that induces ...