designed to deliver unmatched accuracy and sensitivity in spot detection. We paired our novel detection algorithm with the most intuitive software available. Simply load your plate, hit "Read," and get your results. With ASTOR 2, it's not just about reading spots – it's about pioneering a ...
TheAIDiSpotReadercan switch betweenbothapproaches without hardware changesandwithout loss ofsensitivityin the assay analysis. Thesophisticatednewoverlay-technologyallows for detailedinterpretationofdouble-expressing T-cells inevery well. Theadaptedfluorescentinterpretation software especiallyfacilitatesworkingwith theses...
–ELISPOT plate reader or dissecting microscope for visual inspection Reagent preparation •ELISA/ELISPOT Coating Buffer (1X)Make a 1:10 dilution of 10X PBS ELISPOT Coating Buffer in dH2O or reconstitute ELISA/ELISPOT Coating Buffer Powder to 1L in dH2O and sterilize by filtering using a 0.22-...
Also, we developed an ELISpot plate reader-free method to detect and quantify spots, which we compared to manual spot counting and automated analysis by an ELISpot plate reader. We observed strong SARS-CoV-2-reactive T cell responses in COVID-19 convalescent, and COVID-19 vaccinated volunteers...
The AID ELISPOT plate reader software used to determine frequency of spots also has the capability to determine the size of each spot. Consideration of spot size in combination with spot forming units was investigated in our studies of BCG immunogenicity. This additional readout was found to ...
2.2. IFN-γ Assay and Reference Plate Human Interferon-γ ImmunoSpot®Kits (Cat # CTL-H1FNG-1/5M Cellular Technology Limited, OH, USA) were used to perform the ELISPOT assays. The assays were performed according to manufacturer’s instructions. Briefly, the PVDF membranes were coated with ca...
ELISPOT plates were scanned and analyzed using an ImmunoSpot® Reader by CTL. Spots were counted automatically using the ImmunoSpot® 5.041 Software (Basic Count™ mode) for each antigen stimulation condition and the medium negative controls. The counted spots were reported as mean spot-forming ...
For the 384-well plate, all other reagents used were also used at one-third the volumes used in the 96-well plate. The results of both the 96- and 384-well plate assays were counted using the ImmunoSpot® Software (version 5.3.6) which includes a 384-well plate counting suite. As ...
ELISPOT plates were counted with an ImmunoSpot® S6 Ultimate Reader by CTL. SFU were calculated automatically by the ImmunoSpot® Software (Version 7, CTL, Shaker Heights, OH, USA) using the AutogateTM function [14]. ELISPOT assays measuring human IL-1β, IL-6, IL-10, IL-12 and TNF...
International Journal of Molecular Sciences Article The Cytomegalovirus-Specific IL-21 ELISpot Correlates with Allograft Function of Kidney Transplant Recipients Monika Lindemann 1,* , Johannes Korth 2, Ming Sun 2 , Shilei Xu 2, Christoph Struve 2, Kai Werner 2, Theresa Dornieden 1, Peter A. ...