3.8. Discussion The immune monitoring world has been alarmed by a proficiency panel report which made the observation that when analyzing the same PBMC samples, different labs provide different results for T cell assays leading to any comparison between them meaningless [2]. In follow-up studies,...
By introducing 384-well ELISPOT assays, we have been able to reduce the number of PBMC required by three-fold, corresponding to the three-fold reduction in membrane surface area for the 384-well plate. A systematic side by side comparison of the two assay formats showed that antigen-induced ...
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