Synthesize one peptide (up to 22aa,75%); Conjugate the peptide to carrier protein (KLH, BSA or OVA); Immunize two New Zealand rabbits; Titer the immunization by ELISA; Post-bleed collection.1-2ml pre-bleed 1-5mg antibody against unmethylated peptide 1-5mg Lys(Me3) specific antibody Project...
将特异性抗原包被在固相载体上,加入含待测抗体的样品,使之与固相抗原结合(Ag-Ab),再加入酶标记的抗抗体(亦称二抗),与上述Ag-Ab复合物结合形成Ag-Ab-α-Ab-E复合物。此时加入底物,复合物上的酶则催化底物而显色,以酶与底物的显色反应程度来确定待测抗体的效价。由于每步之间均有冲洗步骤,因此若样品中不含相...
ELISA antibody titer in time-point serum samples from participants in 20 and 50 µg groups.Jinhong HuZhihui ChenJun GuMobin WanQian ShenMariePaule KienyJia HeZhen LiQingfeng ZhangZarifah Hussain Reed
鸡热休克蛋白70酶免试剂盒,(HSP-70)ELISA检测试剂盒 ,英文名:HSP-70 ELISA Kit ,英文缩写:HSP-70 ,常见试剂盒规格:96T/48T,产品别名:鸡热休克蛋白70ELISA检测试剂盒,HSP-70 检测试剂盒, HSP-70 检测试剂盒 ELISA实验操作较繁杂,操作不当将引起较大的误差。鸡热休克蛋白70酶免试剂盒,(HSP-70)ELISA检测试...
(3) Preparation of Lincomycin Rabbit Polyclonal Antibody A New Zealand rabbit is selected as the animal for immunization and the lincomycin hapten-carrier protein conjugate is used as the immunogen. The antibody titer in serum is detected after several immunizations. When the titer is higher than ...
Ag-Dab cross-reactivity is not necessarily a problem and can even be used to one’s advantage, as the cross-reacting detection antibody can be used as an additional detection for the targeted antigen. Capture Antibody-Conjugate Cross-Reactivity: The conjugate, such as SHRP, nonspecifically ...
1.A biotin-conjugated monoclonal antibody to AAV8 (clone ADK8)is bound to the immune complex.2.A streptavidin peroxidase conjugate reacts with the biotin molecules.Addition of substrate solution results in a color reaction,which is proportional to the amount of specifically bound viral particles....
The widespread popularity of porcine circovirus type 2(PCV2) has seriously affected the healthy development of the pig industry and caused huge economic losses worldwide. A rapid and reliable method is required for epidemiological investigation and evalu
Results of minimum and maximum anti-rabies antibody titer measured by RFFIT and ELISA methods in patients group according to their specific medical conditions.Pooneh RahimiRouhAllah VahabpourMohammad Reza AghasadeghiSyed Mehdi SadatNad...
Associations between IgG antibody avidity and total IgG ELISA titer.Sumi, BiswasPrateek, ChoudharySean, C. EliasKazutoyo, MiuraKathryn, H. MilneSimone, C. de CassanKatharine, A. CollinsFenella, D. HalsteadCarly, M. BlissKatie, J. Ewer...