Enzyme-linked immunosorbent assay (ELISA): Antibody responses were determined by measuring binding of plasma IgG to recombinant HIV-1 SF162 gp140 trimer. The gp140 trimer was produced as described60 by transient transfection in Expi293F cells and purified over a Galanthus nivalis lectin-coupled ag...
A) Titration curve of humAb binding to HBsAg in an ELISA.Antonella CerinoCorinna M. BremerDieter GlebeMario U. Mondelli
3. Titration des Challenge-Virus Die Verdünnung des verabreichten BRSV-Challenge-Virus (BRSV-Virus zur Belastungsinfektion) wurde reihenverdünnt und auf MDBK-Zellen in 96-Well-Mikrotiterplatten inokuliert. Das Vorliegen des Virus wurde vom Vorliegen des zytopathischen Effekts angezeigt und ...
Serum samples were collected before and during immunization, and antibody titres against SARS-CoV-2 proteins were monitored using ELISA and CPE-VNT. Similar to the observations in mice, the titration of horse plasma samples verified the effectiveness of the immunization schedule, as the highest antib...
In the WHO Manual for the virological investigation of polio, titration of sera from 8 to 1024 is recommended [1]. Therefore, in practise, this is a titre of 8. According to this study, all adults were protected against the poliovirus type 1 Sabin strains. In contrast, one-fifth of the...
Assay procedure 1.Number: to sample correspondmicrotitration well andNumber Sequence, each plate should be setfemininecomparison 2 wells, masculine comparison 2 wells, blank comparison 1 well(don’t add sample andHRP-Conjugate reagent toblank comparison well, other each step the operationaresame). ...
价格(CNY)/ 1 each ERR#1200 - 本商品不能在线订购。 AB-TUBE-ANTI-V5(PK) 规格 Unit Size1 each 文件和下载 证书 搜索 批号Certificate TypeDateCatalog Number(s) 2986799Certificate of Analysis2024年9月27日460705 2901472Certificate of Analysis2024年6月15日460705 ...
本试剂盒采用双抗原夹心酶联免疫法(ELISA)测定标本中人单纯疱疹病毒Ⅰ型抗体(HSVⅠ-Ab)。用纯化的人单纯疱疹病毒Ⅰ型抗体(HSVⅠ-Ab)抗原包被微孔板,制成固相抗原,可与样品中单纯疱疹病毒Ⅰ型抗体(HSVⅠ-Ab)相结合,经洗涤除去未结合的抗体和其他成分后再与HRP标记的单纯疱疹病毒Ⅰ型抗体(HSVⅠ-Ab)抗原结合,形成...
100 μl of each supernatant were tested in an enzyme-linked immunosorbant assay (ELISA) specific for mouse IL-2 and mouse IFN-γ (Mabtech, Nacka Strand, Sweden). The hybridoma clones tested positive were re-tested on target cells loaded with CINGVCWTV (SEQ. ID. NO.: 10) [1073-1081]...
猪圆环抗体(PC-Ab)试剂盒使用实验原理本试剂盒应用双夹心法测定中。用纯化的包被微孔板,制成固相抗,,经过彻底洗涤后底物TMB显色。TMB在酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。用酶标仪在nm波长下测定吸光度(OD值)试剂盒组成 酶板稀释液-20℃保存,但应避免反复冻融操作步骤 μl。然后在待测样...