The elastin-like protein polypeptide-coxsackie adenovirus receptor (ELP-CAR) fused protein is composed of ELP, a spacer region and a CAR-D1 region. The preparation method of the elastin-like protein polypeptide-coxsackie adenovirus receptor fused protein comprises the following steps: constructing an...
This approach was justified by the observation that buried hydrophobic turns are commonly observed to interact in the Protein Data Bank. After dimerization, the ensemble-averaged hydrodynamic properties were calculated for each degree of -turn bias, and the results were compared with analytical ...
Cells on the ELP-RGD gel showed four times higher gene expression of the contractile protein troponin T type 2 than those on a Col-I gel, which is an effective substrate for iPSC cardiac differentiation. The ELP-RGD gel might stimulate integrin-derived signaling pathways in the cells to ...
Proteins and synthetic polymers that undergo aqueous phase transitions mediate self-assembly in nature and in man-made material systems. Yet little is known about how the phase behaviour of a protein is encoded in its amino acid sequence. Here, by synthesizing intrinsically disordered, repeat protei...
For the first time, they have demonstrated C-terminal fusion of IFN-α to an elastin-like polypeptide (ELP) to form a well-defined IFN-ELP fusion protein that was long acting and highly potent for cancer therapy. IFN-ELP fusion protein can be easily produced in E. coli with high yield ...
Recombinant elastin-like protein (ELP) polymers display several favorable characteristics for tissue repair and replacement as well as drug delivery applications. However, these materials are derived from peptide sequences that do not lend themselves to cell adhesion, migration, or proliferation. This re...
Elastin-like polypeptides revolutionize recombinant protein expression and their biomedical application - ScienceDirect DM Floss,S Kai,S Rose-John,... 被引量: 0发表: 2010年 Unclogged pores: designer channels for protein translocation Nanopores have the potential to revolutionize the field of protein ...
Costa2, Susana R. Chaves1, Raul Machado1, Margarida Casal1 & Tony Collins1 Silk-elastin-like proteins (SELPs) are a family of genetically engineered recombinant protein polymers exhibiting mechanical and biological properties suited for a wide range of applications in the biomedicine...
This research project primarily focuses on the application of elastin-like polypeptides (ELPs) as intracellular protein switches. ELPs are biologic, environmentally responsive polymers comprised of repeats of the Val-Pro-Gly-Xaa-Gly motif, a sequence derived from the gene for human tropoelastin. Mod...
In fact it is recommended that each and all of these parameters be optimised for each particular expression system, protein product and cultivation mode used. As a first step in our undertaking to study and optimise the production levels of the novel SELPs, we investigated batch fermentation in...