Silk-elastin-like proteins (SELPs) are a family of genetically engineered recombinant protein polymers exhibiting mechanical and biological properties suited for a wide range of applications in the biomedicine and materials fields. They are being explored as the next generation of biomaterials but low ...
Cells on the ELP-RGD gel showed four times higher gene expression of the contractile protein troponin T type 2 than those on a Col-I gel, which is an effective substrate for iPSC cardiac differentiation. The ELP-RGD gel might stimulate integrin-derived signaling pathways in the cells to ...
We describe the fabrication of thin lamellae consisting of continuous collagen microfiber embedded at controlled orientations and densities in a recombinant elastin-like protein polymer matrix. Multilamellar stacking affords flexible, protein-based composite sheets whose properties are dependent upon both ...
This approach was justified by the observation that buried hydrophobic turns are commonly observed to interact in the Protein Data Bank. After dimerization, the ensemble-averaged hydrodynamic properties were calculated for each degree of -turn bias, and the results were compared with analytical ...
正在加载 Silk-elastinlike protein polymers for matrix-mediated cancer gene therapy. Adv. Drug Deliver. Rev. Pub Date : 2010 Dec 3DOI : 10.1016/j.addr.2010.04.006 Joshua A. Gustafson , Hamidreza Ghandehari Silk-elastinlike protein polymers (SELPs) are recombinant polymers designed from silk ...
Silk-elastinlike proteinnanogelthermoresponsivedrug deliverymatrix-metalloproteinaseSilk-elastinlike protein polymers (SELPs) self-assemble into nanostructures when designed with appropriate silk-to-elastin ratios. Here, we investigate the effect of insertion of a matrix metalloproteinase-responsive peptide ...
In fact it is recommended that each and all of these parameters be optimised for each particular expression system, protein product and cultivation mode used. As a first step in our undertaking to study and optimise the production levels of the novel SELPs, we investigated batch fermentation in...
In this study, a polypeptide fusion strategy was applied to explore the capacity of elastin-like polypeptide (ELP) and γ-zein fusions in increasing the accumulation of the recombinant protein in soybean seeds. Transgenic soybean plants were generated to express the γ-zein- or ELP-fused green ...
Elastinisamajorproteincomponentofthevascularwallandisrespon- sibleforitsunusualelasticproperties.PolymersofitsrepeatingVPGVG sequenceshavebeensynthesisedandshowntoexhibitaninversetem- peraturetransitionwhere,astemperaturerises,thepolymercollapses fromanextendedchaintoab-spiralstructurewiththreeVPGVGunits perturn,eachpen...
Purification of recombinant proteins is performed by expressing in a host cell a fusion protein comprising: (a) a product protein domain, (b) an intein, and (c) at least one aggrega