Exogenous biotin intake is becoming a common cause of apparent abnormal thyroid function tests in medical practice. Case Report: A 50-year-old lady came to the medical OPD with reports of a "whole body test profile" which showed low TSH andhighT3 and T4. She had no sympto...
Therefore, DE has a protective effect on common carp under acute ammonia exposure. Optimal doses of DE can improve the survival rate, antioxidant capacity, and nonspecific immunity, while can reduce the stress and inflammatory reaction of common carp caused by acute ammonia exposure. The second-...
DNA was end labeled with biotin (Thermo Scientific, USA), purification using QIAquick Kit (QIAGEN) and 20 fmol/reaction of the labelled DNA was incubated in binding buffer (10 mM Tris-HCl pH 7.5, 40 mM NaCl, 5% glycerol, 1 mM EDTA), BSA (6 μ g), poly (dIdC) (1...
Furthermore, on one section per animal the antiprolif- erating cell nuclear antigen (PCNA, BioGenex, San Ra- mon, CA) immunocytochemical reaction was performed to detect mitotic processes. The reaction was revealed by a biotin-streptavidin-alkaline phosphatase supersensitive detection system (BioGenex...
Curcumin has been used in traditional Indian medicine for the treatment of many diseases, such as wounds, arthritis, trauma, ulcers, fever and skin diseases, over the past 60 years4,5. Furthermore, curcumin exerts beneficial effects on cardiovascular disease, although the molecular targets are ...
Yuling Wei,Yang Yang, Chaonan Ji, Xinyue Hu, Shaoshan Mai, Shengnan Kuang, Xiaoyan Tian,Ying Luo, Guojuan Liang & Junqing Yang In the present study, the agonists and antagonists of DP receptor were used to examine whether the PGD2-DP signaling pathway affects ...
Our results suggest that combined therapy to enhance axonal plasticity based on TST and 5-Aza-dC constitutes a promising approach for promoting the recovery of function in the chronic stage of stroke. Keywords: stroke; chronic stage; task-specific training; DNA methylation; axonal plasticity; ...
of diet: vitamin D3 2200 IU; vitamin E 220 mg; vitamin K3 1 mg; vitamin B1 20 mg; vitamin B2 10 mg; vitamin B6 10 mg; vitamin B12 0.1 mg; nicotinic acid 40 mg; pantothenate 22 mg; folic acid 1 mg; biotin 1 mg; choline chloride 400 mg; vitamin C 120 mg; Cu 20 mg; Fe ...
The 10 µg nuclear protein was incubated with a buffer containing 10 mM Tris, 5 mM MgCl2, 50 mM KCl, 1 mM DTT, 2 µg poly (dI·dC), and 2 pmol biotin- labeled, double-stranded NF-κB or AP-1 oligonucleotides probes (Promega, Madison, WI, USA) at room temperature for 20 ...
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