EF1-alpha promoter versions of the Tet-On 3G inducible expression system provide for consistent long-term expression of the Tet-On 3G transactivator, even in cell types known for their tendency to silence a CMV promoter over time, such as hematopoietic cells and stem cell...
Ueda, Y., Mizuno, N., and Araki, M., Transgenic Xenopus laevis with the ef1 α Promoter as an Experi mental Tool for Amphibian Retinal Regeneration Study, Genesis, 2012, doi: 10.1002/dvg.22024.Ueda Y, Mizuno N, Araki M (2012) Transgenic Xenopus laevis with the ef1-alpha promoter as ...
(EF1alpha, Bicistronic) is a powerful, tightly regulated, tetracycline-inducible mammalian expression system that is controlled by the addition of doxycycline to the culture medium. This system allows the simultaneous expression of two genes of interest under the control of a Tet-responsive promoter ...
including primary and stem cells. The vector allows a gene-ofinterest to be fused to the C-terminus of the red fluorescent protein mCherry. Expression of the fusion is driven by the human elongation factor 1 alpha (EF1α) promoter, which continues to be constitutively active even after stable...
(2012). Generation of a genome scale lentiviral vector library for EF1alpha promoter-driven expression of hu- man ORFs and identification of human genes ... ?kalamera Dubravka,D Mareike,AS Purdon,... - 《Plos One》 被引量: 24发表: 2012年 Generation of a Genome Scale Lentiviral Vector Libr...
(EF1a) promoter is a strong mammalian expression promoter derived from the elongation factor 1 alpha (EF1A) gene, which can stably drive the constitutive expression of its downstream genes in a variety of cells, including stem cells, primary cells, hematopoietic cells, etc.T2A is a 2A peptide ...
USE OF PROMOTER OF EF1α GENE FOR EXPRESSING FOREIGN DNA IN CHRYSANTHEMUM 来自 百度文库 喜欢 0 阅读量: 5 申请(专利)号: JP20020228576 申请日期: 2002-08-06 公开/公告号: JP2004065096A 公开/公告日期: 2004-03-04 申请(专利权)人: NATIONAL AGRICULTURE & BIO-ORIENTED RESEARCH ORGANIZATION ...
stable integration of the vector into the host cell genome. Stable, constitutive expression of the fusion protein is driven by the human elongation factor 1 alpha (EF1α) promoter, allowing the monitoring of a variety of cellular processes (such as differentiation in primary or stem cells)withou...
Vip3Agene,EF1αPromoterand TransformationinTobacco AThesisSubmittedtoChongqingUniversityinPartial FuLfillmentoftheRequirementforthe DegreeofMasterofScience By MaZuo-Jiang Supervisor:Prof.LiZheng-Guo Major:botany CollegeofBioengineeringofChongqingUniversity,
IL-6 promoter-Luc-EF1α-mCherry Hela Cells,即IL-6 promoter-Luc-EF1α-mCherry-T2A-Puro Hela细胞,是一种细胞基因组中含有多个IL-6 promoter结合位点的多克隆Hela稳定细胞株(Polyclonal Hela stable cell line),通过检测萤火虫萤光素酶(Firefly luciferase, Luc)的活性,可以高灵敏度地检测IL-6 promoter的激活...