EF1-alpha promoter versions of the Tet-On 3G inducible expression system provide for consistent long-term expression of the Tet-On 3G transactivator, even in cell types known for their tendency to silence a CMV promoter over time, such as hematopoietic cells and stem cells...
including primary and stem cells. The vector allows a gene-ofinterest to be fused to the C-terminus of the red fluorescent protein mCherry. Expression of the fusion is driven by the human elongation factor 1 alpha (EF1α) promoter, which continues to be constitutively active even after stable...
IL-6 promoter-Luc-EF1α-mCherry A549 Cells,即IL-6 promoter-Luc-EF1α-mCherry-T2A-Puro A549细胞,是一种细胞基因组中含有多个IL-6 promoter结合位点的多克隆A549稳定细胞株(Polyclonal A549 stable cell line),通过检测萤火虫萤光素酶(Firefly luciferase, Luc)的活性,可以高灵敏度地检测IL-6 promoter的激活...
This LV-EF1α-Cre-Puro pre-madelentivirus expresses Cre recombinase with co-expression of puromycin selection in an IRES cassette under EF1αpromoter. Ready to use format. Titer:> 1x109TU/mL Selection Antibiotic:Puromycin Purity*:In vivo grade and OK for both in vitro tissue culture infection...
(EF1a) promoter is a strong mammalian expression promoter derived from the elongation factor 1 alpha (EF1A) gene, which can stably drive the constitutive expression of its downstream genes in a variety of cells, including stem cells, primary cells, hematopoietic cells, etc.T2A is a 2A peptide ...
Description: LV-EF1α-mCherry-Puro is a pre-made lentivirus which express mCherry under the EF1α promoter with co-expression of puromycin selection in a IRES cassette. Ready to use format. Titer: > 1x109 TU/mL Selection Antibiotic: Puromycin Purity*: In vivo grade and OK for both in ...
(2012). Generation of a genome scale lentiviral vector library for EF1alpha promoter-driven expression of hu- man ORFs and identification of human genes ... ?kalamera Dubravka,D Mareike,AS Purdon,... - 《Plos One》 被引量: 24发表: 2012年 Generation of a Genome Scale Lentiviral Vector Libr...
Expression System is a powerful, tightly regulated, tetracycline-inducible mammalian expression system that is controlled by the addition of doxycycline to the culture medium. In the EF1α Version, Tet-On 3G transactivator expression is driven by a human elongation factor 1 alpha (EF1α) promoter,...
stable integration of the vector into the host cell genome. Stable, constitutive expression of the fusion protein is driven by the human elongation factor 1 alpha (EF1α) promoter, allowing the monitoring of a variety of cellular processes (such as differentiation in primary or stem cells)withou...
Vip3Agene,EF1αPromoterand TransformationinTobacco AThesisSubmittedtoChongqingUniversityinPartial FuLfillmentoftheRequirementforthe DegreeofMasterofScience By MaZuo-Jiang Supervisor:Prof.LiZheng-Guo Major:botany CollegeofBioengineeringofChongqingUniversity,