11a), we show that overexpression of wild-type eEF1A1 also strongly decreases the activation of the Mbp promoter (Supplementary Fig. 11i). Taken together, these data show that Ac-eEF1A1 is deacetylated by HDAC2 and negatively regulates the expression of Sox10 and of the Sox10 target genes...
Meanwhile, it is verified that ARID3A is a transcriptional regulator of enolase 2 (ENO2), an important enzyme in the glycolytic process. Nef suppresses ENO2 transcription activation by affecting ARID3A binding to the promoter region of ENO2, which results in macrophage glycolytic reprogramming ...
To examine the importance of the UTR of eEF1A1 mRNA, we generated an expression vector for destabilized enhanced green fluorescent protein (d2EGFP), having an approximately 2-hour half-life in vivo, driven by the eEF1A1 promoter (EF). In this system, transcribed d2EGFP mRNA contains the ...
Function:This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate ...
We previously showed that miR-122 was frequently downregulated in hepatocellular carcinoma (HCC) and C/EBPα transactivated miR-122 expression. In this study, we found that Sp1 bound to the miR-122 promoter at two different sites. Interestingly, either inhibition or overexpression of Sp1 could de...
同时在大鼠心梗后,无论是靶向成纤维细胞(Postn promoter-FMO2)还是靶向非心肌细胞(miR133a/miR1a TS-FMO2)补偿FMO2,均能够明显抑制心梗后的纤维化表型,并且改善大鼠的心脏功能。此外,研究者进一步在非人灵长类动物的心梗模型中过表达...
The growth promoter spermine interacts specifically with dermatan sulfate regions that are rich inl-iduronic acid and possess antiproliferative activity We have investigated interactions between spermine, a member of the growth promoting polyamine family, and various glycosaminoglycans. By using gel chromat...
STAT1 increases the transcriptional activity of cyclin D1 by binding to the cyclin D1 promoter. Taken together, these findings enabled us to identify a novel mechanism by which eEF1A1 regulates the cell cycle's G1 phase to promote tumor proliferation by regulating cyclin D1 expression through ...
Further investigations disclosed that Sp1 cooperated with C/EBPα to induce miR-122 transcription by binding to the positive regulatory site D in the miR-122 promoter, whereas eEF1A1 interacted with Sp1 to bind to the negative regulatory site E and inhibit miR-122 transcription. Significantly, ...
Notably, TOPK phosphorylated YB1 at Thr 89 (T89) and Ser 209 (S209) amino acid residues, then the phosphorylated YB1 bound with the promoter of the eukaryotic translation elongation factor 1 alpha 1 (eEF1A1) to activate its transcription. Consequently, the AKT/mTOR signal pathway was ...