Previously, we identified 2 ecdysteroid kinase-like (EcKL) genes-Wallflower (Wall) and Pinkman (pkm)-in the model fruit fly Drosophila melanogaster that are orthologs of the ecdysteroid 22-kinase gene BmEc22K. Here, using gene knockdown, knockout, and misexpression, we explore Wall and pkm's ...
Abstract Ecdysteroids are polyhydroxylated sterols, and are widespread in the plant and animal world. To date, over 520 ecdysteroids have been isolated from natural sources. The most accessible and studied representative of this class of compounds is 20-hydroxyecdysone (20E)—a universal hormone for...
The lKCs show preferential expression of five genes encoding proteins involved in Ca2+-signaling, such as Ca2+/calmodulin-dependent protein kinase II (CaMKII)19, which plays a central role in the synaptic plasticity that serves as a platform for learning and memory processes in many animal ...
Ecdysterone also causes an influx of calcium ions into the cells, which causes an increase in the phosphorylation of Akt. Akt is a protein kinase that mediates protein synthesis, so if ecdysterone increases its activity, then this could be the mechanism through which it enhances muscle growth.[...
To avoid the further confusing numberings to represent the same functional orthologues among insect species, we would like to propose a unique subfamily name, noppera-bo (nobo), for these orthologues. Hereafter, we refer to B. mori GSTe7 as nobo-Bm in this manuscript. Table 4 | Rescue of...
Ecdysteroid kinase-like (EcKL) paralogs confer developmental tolerance to caffeine in Drosophila melanogasterdoi:10.1101/2021.09.15.460555Jack L. ScanlanPaul BattlayCharles RobinCold Spring Harbor Laboratory
Identifying candidate detoxification genes in the ecdysteroid kinase-like (EcKL) and cytochrome P450 gene families in Drosophila melanogaster by integrating evolutionary and transcriptomic datadoi:10.1101/2020.02.17.951962Scanlan JlGledhill-Smith RsBattlay P...
(STAT),which is a key component in the Janus-activated kinase / STAT pathway.Luciferase activity assays showed that two CREs enhanced the transcriptional activity ofBmIGFLP.Electrophoretic mobility shift and chromatin immunoprecipitation assays demonstrated that BmSTAT proteins in the nuclear extracts ofB...
Recombinant MIR extracts from a baculovirus expression system contained high constitutive kinase activity in the presence of manganese or magnesium. Activation was independent of a ligand. SDS-gel analysis suggested that the recombinant receptor was not post-translationally processed into an 伪 and 尾-...
The recent rediscovery that in both invertebrates and vertebrates at least some isoforms of Ca2+-ATPase need the presence of an endogenous farnesol/juvenile hormone(JH)-like sesquiterpenoid for keeping cytosolic [Ca2+]i below the limit of apoptosis-induction, triggered the idea that it is not ...