In addition to compounds with ecdysteroid-like activity, compounds which block the hormonal action of ecdysteroids (antagonists) may also have a natural role in insect–plant relationships or find application as
The glutathione S-transferase (GST) fusion protein system is widely used for high-level expression and efficient purification of recombinant proteins from bacteria. The goal of this study was to clone, efficiently express and purify the ecdysteroid-regulated protein (ERP) in the form of a GST ...
Bownes, M., Scott, A., and Blair, M. (1987). The use of an inhibitor of protein synthesis to investigate the roles of ecdysteroids and sex determination genes on the expression of the genes encoding theDrosophilayolk proteins. Development101, 931–941. PubMedCASGoogle Scholar Bownes, M.,...
An ecdysteroid-regulated 16-kDa protein homolog (named Pc-E16), encoding 150 amino acid residues with a conserved MD-2-related lipid-recognition domain, was first identified in Procambarus clarkii. Phylogenetic analyses indicated similarity between Pc-E16 and 16-kDa proteins from Aplysia californica ...
E93, the Drosophila orthologue of honey bee Mblk-1 functions in morphogenesis during metamorphosis downstream of ecdysone-signaling via the ecdysone receptor as an ecdysteroid-regulated protein30,38,39,40. In contrast, MBR-1, the Caenorhabditis elegans orthologue of honey bee Mblk-1, is required ...
Cuticle renewal is a complex biological process that depends on the cross talk between hormone levels and gene expression. This study characterized the expression of two genes encoding cuticle proteins sharing the four conserved amino acid blocks of the
In the germband elongation stage, nobo expression was up-regulated in the amnioser- osa (Fig. 1k and Supplementary fig. S1e). At stage 16 and later, nobo expression was detected in the PG cells (Fig. 1l). This expression pattern is reminiscent of some Halloween genes, such as phm and...
Inacute myeloid leukemia(AML), ecdysteroids have been studied as an alternative to steroids like dexamethasone, which are frequently used in combination with chemotherapy and radiation during treatment. 20HE showed positive results inin vitrostudies, decreasing the proliferation of cancer cells, increasin...
The stimulatory effect of HP19 on the ACP activity is developmentally regulated and exhibits its maximal effect shortly before the onset of metamorphosis. We cloned the HP19 cDNA by immunoscreening a hind gut-associated lobular fat body cDNA expression library. Analysis of the amino acid sequence ...
This novel CRC-ECRB2 complex mediates an ecdysteroid signal by activating the expression of ETH and controlling ETH protein levels in the Inka cells. These results demonstrate that cofactors, like CRC, binding the AF1 domain can modify the role of EcRB2 to regulate the expression of selected ...