Here, we present a protocol to culture primary human vascular smooth muscle cells (VSMCs) under Alzheimer's disease (AD)-like conditions, including steps for morphological characterization with microscopy. We then describe functional assays, including wound healing, transwell, coculture, and ...
(D) Canonical gene expression markers for major cell types: CD8T cells (CD3D, CD8A), CD4T cells (CD3D, CD4) natural killer T (NKT) cells (CD3D, NCR3), natural killer (NK) cells (NCR3), monocytes (CD14, FCGR3A [CD16]), dendritic cells (DCs; FCER1A), and B cells (MS4A1)....
We previously demonstrated that chronic lymphocytic leukemia (CLL) cells induce alterations in global gene expression profiles in patient CD4 and CD8 T cells, and a profound T cell immunological synapse formation defect that can be reversed with lenalidomide (J Clin Invest. 2005;115(7):1797-1805,...
Immune imbalance is a common characteristic of patients with chronic lymphocytic leukemia (CLL). This feature is shared with E渭- TCL1 transgenic mice that, like CLL patients, exhibit an expansion of CD5+ B cells with associated non-B-cell defects. In patients and in mice, T-cell responses...
as brown dots in the nucleus and, to a lesser extent, in the cytoplasm of cells mostly localized in the cortical region and in the germinal follicles of the node, especially in the T cell areas as shown by the staining with anti-CD3 and CD20 antibodies for T and B cells (Figure 4)....