device for the automatic selection of images of chromosomes during metaphaseFAVIER CTOURNIER ELE GO R
Metaphase spread and chromosome count Immunofluorescence on metaphasic spread was obtained as previously described with few modifications48. Cultures were treated with 100 ng ml−1 nocodazole for 8 h overnight, collected by trypsinization, resuspended in 0.2% (w/v) KCl and 0.2% (w/v) ...
aSurprisingly, all three were found to be necessary for timely and efficient alignment of the chromosomes during prometaphase. The underlying mechanisms are unknown. 惊奇地,全部三被发现必要的为染色体的实时性和高效率的对准线在prometaphase期间。 部下的机制是未知的。[translate]...
Metaphase I MNase: Micrococcal nuclease MRX: Mre11–Rad50–Xrs2 ssDNA: Single-stranded DNA Pac: Pachytene stage PCA: Principal component analysis RNAPII: RNA polymerase II ssDRIP-seq: Single-strand DNA ligation-based library construction from DNA:RNA hybrid immunoprecipitation, followed by...
In hybrid spermatocytes, a high frequency of asynapsis of X and Y chromosomes during the pachytene-like stage and dissociation of these chromosomes during metaphase I (MI) was observed. No autosomal univalency was observed during pachytene-like and MI stages in the hybrids; however, a low ...
Moreover, PRRC2A-null spermatocytes exhibit delayed metaphase entry, chromosome misalignment, and spindle disorganization at metaphase I and are finally arrested at this stage. Sequencing data reveal that PRRC2A decreases the RNA abundance or improves the translation efficiency of targeting transcripts. ...
(red) were observed on a pair of homologous chromosomes of B73 metaphase cells (a), while absent on Mo17 metaphase chromosomes (b). Only one foci signal can be detected on mitotic metaphase spreads of B73 × Mo17 F1 hybrid (c). Scale bars = 10 μm.dLeft: PAV map of RegionA ...
While depleting Daxx or Atrx had little effect on wild-type J1 cells, TKO cells exhibited increased fragile telomeres, with smeared or double telomere signals on metaphase chromosomes (Figure 5C). Dysregulated telomere length control can result in abnormally long telomeres with average lengths of >1...
To determine whether polyploid recurrent tumor cells have elevated rates of aneuploidy, we performed metaphase spreads on polyploid and diploid cells from the spontaneous and induced model. As expected, the median number of chromosomes in metaphase cells from the polyploid population was approximately ...
‘bamtobed’. Using BEDtools intersect, read counts were obtained for contiguous 50 kb genomic bins. For each cell the average of the bin counts was calculated for chromosomes 1–19; these 19 values were then next used to calculate the coefficient of variation as standard deviation ...