Necroptosis is regulated by various kinases, such as RIP1, RIP3, mixed lineage kinase domain-like protein (MLKL), and PGAM5 [163]. Excessive mPTP opening is a prerequisite for necroptosis [13,164], and Drp1-mediated ROS accumulation is a major trigger of necroptosis in ischemia and hypoxia ...
It inhibits the formation of finger ring-like structures of Drp1 oligomers and suppresses the activity of GTPases.27 To investigate whether EBV-LMP1 promotes mitochondrial division and maintains cell survival by increasing Drp1 activity, we measured cell viability by MTS assay after cells were ...
(b) Quantification of mitochondrial morphology of ∼500 cells per condition: category 1 (elongated tubulin-like structure), category 2 (intermediate length) and category 3 (fragmented mitochondria). *P<0.05 compared with glutamate (5 mM)-treated cells (n=3 independent experiments, ANOVA, ...
Dynasore (Dynamin Inhibitor I) 是一种可渗透细胞的化学物质,可逆且非竞争性地抑制 dynamin 1/2 的 GTPase 活性。
These results indicate that inhibition of Drp-1-mediated mitochondrial fission induces replicative senescence-like phenomena in younger BMSCs, while Drp1 activation partially improves the functions of BMSCs. Thus, Drp1-mediated mitochondrial dynamics are upstream regulators of the senescent state and ...
levels (Fig.3E, F). However, silencing MAP4K4 significantly improved the mitochondrial DNA copy number and CoQ10 levels after HG/FFA injury (Fig.3E, F). Together, the present data strongly indicate that silencing MAP4K4 exerts protective effects on endothelial mitochondrial structure and function...
Previous studies on NP cells showed that soft substrate maintained a juvenile-like state of NP cells, while stiff substrate induced a degenerative-like phenotype [32, 49]. Recently, we found that ECM stiffness activated the mechanosensitive ion channel Piezo1 and resulted in senescence and ...
In muscle, MT form a non-classic grid-like network [37] that was unaffected by Drp1 overexpression (Fig. S3A, B) as well as the actin microfilaments (MF) (Fig. S3A, B), confirming the possibility for mitochondria to move along the cytoskeleton. Consistently, we detected a directional ...
Typically, Drp1 exists in the cytoplasm and translocates to the mitochondrial outer membrane (MOM), where it assembles into a spiral-like structure exerting a mechanical force on the mitochon- dria, leading to constriction and subsequent fission [12]. However, the significance of Drp1 ...
In addition, growth factor erv1-like (Gfer) helps maintain pluripotency in murine ESCs, and preserves survival ability via the modulation of Drp1 [88]. To further clarify the mechanisms of the Gfer-mediated mitochondrial fission process, Todd et al. studied knockdown Gfer in ESCs and found ...