(OPA1)]. Mutations have been identified in the fusion proteins MFN2 and OPA1 causing various diseases. Mutation in MFN2 have been detected in neuropathies, like Charcot–Marie–Tooth type 2 A (CMT2A) and other type of Hereditary Motor and Sensory Neuropathies (HMSNs), though the exact ...
To determine the effect of this small helical segment in L3S on the activity of Drp1, a charge reversal was introduced through a mutation, R456E, to disrupt this lock and promote the opening of the BSE away from the stalk. In a previously published structure, GMP-PCP-bound complex of Drp...
2n). After mutation of S637 (to Ala) in CNE1-LMP1 cells, the glycolysis flux could not be reduced by LMP1 knockout (Fig. 2o). Taken together, these results show that glycolysis is closely related to mitochondrial fission, which depends on the phosphorylation of Ser616 and the ...
MutationMolecular StructureAmino Acid SequenceMolecular Sequence DataMammalian Drp1 is a dynamin-like GTPase required for mitochondrial fission. Although it exists primarily as a cytosolic homo-tetramer in vivo, it can also self-assemble into higher order structures on the mitochondrial outer membrane, ...
Therefore, to evaluate the regulation and functional consequences of Drp1 phosphorylation sites in vivo, we generated a whole-body Drp1 KI mutant mouse model harboring a site-specific S to A mutation at S600 (Figure 3A; see STAR Methods). The Drp1 KI mice were viable and fertile, and they ...
AMPK; AMP-activated protein kinase, Drp1; Dynamin-related protein 1, ERK1/2; Extracellular signal-regulated kinases 1 and 2, MyD88; myeloid differentiation primary response 88, JAK; Janus kinase, TLR4;Toll-like receptor 4. STAT3; Signal transducer and activator of transcription 3. 2. ...
Recently, we described thelucmice, carrying a naturally occurring recessive mutation onZdhhc133. This loss-of-function allele (Zdhhc13luc) was confirmed as a nonsense substitution, resulting in a premature stop codon (L203X)3. Phenotypically, homozygousZdhhc13luc/Zdhhc13luc(luc/luc, luc, homozygo...
Although initial analysis indicates that mitochondrial recruitment of ectopically expressed Drp1 in response to staurosporine is unaffected by loss of SUMOylation, we find that Drp1 SUMOylation is enhanced in the context of the K38A mutation. This dominant-negative mutant, which is deficient in GTP...
et al. A lethal de novo mutation in the middle domain of the dynamin-related GTPase Drp1 impairs higher order assembly and mitochondrial division. J Biol Chem 285, 32494–32503 (2010). Article CAS Google Scholar Pistor, S., Chakraborty, T., Niebuhr, K., Domann, E. & Wehland, J. ...
Supporting this, expression of Drp1 containing the phosphomimetic S579D mutation (Drp1S579D) rescued OSKM-induced mitochondrial fragmentation in cells incubated with the MEK inhibitor, whereas expression of the Drp1WT did not (Fig. 5d). Although a contribution of Cdk1 to the phosphorylation of ...