22. We then confirmed the size of these chromosomes using flow cytometry (Extended Data Fig.1g) and mitotic neuroblast spreads (Extended Data Fig.1h). We found that
Flow cytometry First, capillary leukocytes were removed from the liver tissue by perfusion with PBS. Then, the liver tissue was digested at 37 °C for 45 min using Collagenase type 4 (LS004189, Worthington, Lakewood, NJ, USA). Afterwards, the liver was minced through a 70 µm ce...
Sperm surface C inhibitors were quantitated by indirect immunofluorescence flow cytometry using murine monoclonal antibodies to detect C3 regulatory proteins, C3b/C4b receptor type (CRI, CD35), membrane cofactor protein (CD46), and decay-accelerating factor (CD55), and C5b-9 inhibitor, P18 (CD59...
Mouse monoclonal antibodies (mAbs) were developed against Streptococcus pneumoniae in search for potential common pneumococcal proteins as vaccine antigens. mAb 230,B-9 (IgG1) reacted by immunoblotting with a 70-kDa protein which was isolated by immunoaffinity chromatography and subsequent preparative ...
While not tracked in either of these studies, alloantibodies may have been produced; however, further research is needed to understand whether these may have adverse effects on patient outcomes or side effects for consideration of repeat injection, dosing and use of pooled allogeneic versus single ...
The genome size ofB. hybridumBhyb26 was determined by flow cytometry. Sixteen Bhyb26 individuals, grown from seeds, were analysed and each sample was measured twice. All analyses were performed by the same operator when the plants were 4–5 weeks old; a maximum of five plants were analysed...
The role of CCR5 chemokine ligands and antibodies to CCR5 coreceptors in preventing HIV infection HIV infection is transmitted predominantly through mucosal tissues that favour strains of HIV-1 that use CCR5. A lack of cell-surface expression of CCR5 fo... T Lehner - 《Trends in Immunology》 ...
In contrast, INF-γ licensing in human ATMSCs did not induce change in migratory abilities during the scratch assay and VCAM-1 expression by flow cytometry, compared with unlicensed ATMSCs [6]. In addition, some cell adhesion-related genes (CD31 and E-cadherin) of MSCs were expressed at ...
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use in the therapy of cancer in 1997,11 hundreds of mAbs, including murine, chimeric, humanized, and human, have been available for clinical use in everyday practice as a monotherapy, in combination with standard chemotherapy or for theranostic application as radiolabeled monoclonal antibodies.12...